概述（Summary）icon

英文全称

Recombinant Human UBE2S protein

纯度（Purity）

>90% as determined by SDS-PAGE

内毒素（Endotoxin level）

Please contact with the lab for this information.

蛋白构建（Construction）

A DNA sequence encoding the human UBE2S (Met1-Ala209) was fused with His tag

Accession #

Q16763

表达宿主（Host）

E.coli

种属（Species）

Homo sapiens (Human)

预测分子量（Predicted Molecular Mass）

23.21 kDa

制剂（Formulation）

Supplied as solution form in PBS pH 7.5./ Lyophilized from PBS pH 7.5.

运输方式（Shipping）

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

稳定性&储存（Stability &Storage）

Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.

复溶（Reconstitution）

Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.

背景（Background）icon

背景介绍

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A . In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

分子别名（Alternative Names）

Ubiquitin-conjugating enzyme E2 S,E2 ubiquitin-conjugating enzyme S,E2-EPF,Ubiquitin carrier protein S,Ubiquitin-conjugating enzyme E2-24 kDa,Ubiquitin-conjugating enzyme E2-EPF5,Ubiquitin-protein ligase S.