|抗体名：||英利昔单抗 （Infliximab）||浓度：||1mg/ml %|
|纯度：||95% HPLC %||产地：||中国|
|形态：||保存条件：||store at -80°C|
PBS buffer PH7.5
Please contact with the lab for this information.
Infliximab is a humanized antibody against tumor necrosis factor α (TNF-α) that is used in the treatment of Crohn's disease and rheumatoid arthritis.Infliximab is a IgG1κ monoclonal antibody that binds to soluble and transmembrane forms of TNF-α with high affinity to disrupt the pro-inflammatory cascade signalling.Binding of the antibody to TNF-α prevents TNF-α from interacting with its receptors.Infliximab does not neutralize TNF-β (lymphotoxin-α),a related cytokine that utilizes the same receptors as TNF-α Label.Blocked actions of TNF-α further leads to downregulation of local and systemic pro-inflammatory cytokines (i.e. IL-1, IL-6),reduction of lymphocyte and leukocyte migration to sites of inflammation, induction of apoptosis of TNF-producing cells (i.e. activated monocytes and T lymphocytes),increased levels of nuclear factor-κB inhibitor, and reduction of reduction of endothelial adhesion molecules and acute phase proteins 3. Its inhibitory actions on TNF-α was demonstrated in human fibroblasts,endothelial cells, neutrophils, B and Tlymphocytes and epithelial cells Label.Infliximab also atteunates the production of tissue degrading enzymes synthesized by synoviocytes and/or chondrocytes. According to a transgenic mice study that developed polyarthritis due to consitutive levels of human TNF-α,infliximab decreased synovitis and joint erosions in collagen-induced arthritis and allows eroded joints to heal Label.
靶点;物种(Specificity target name;species)
活性研究（体外/体内研究）(Activity in vitro)
To some of the polyclonally or antigen-specific-stimulated cell cultures, 1, 10 or 100 lg/ml of infiximab (MSD, Whitehouse Station, NJ) was added at the time for T cell stimulation or 48 h after T cell stimulation. Consecutive cell culture supernatants were collected and stored every 24 h or at the end of the cell culture. The T cell proliferation was measured at the end of cell culture by pulsing the cells with 0.5 lCi of [3H] thymidine/well (Amersham, Arlington Heights) for the last 8 h, and the incorporated radioactivity was analysed using a scintillation counter.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Store at +4°C short term (1-2 weeks).
Store at -20 °C 12 months.
Store at -80°C long term.
For research use only .