Ferulic acid methyl ester (25 μg/mL) has no cytotoxic effects on BMDMs after treatment for 6 h, 18 h, 48 h^[2] .
Ferulic acid methyl ester (Methyl ferulate; 5, 10, 25 μg/mL) suppresses TNFα, IL6, IFNγ but not IL10, inhibits NO generation at 10 and 25 μg/mL, in primary bone marrow derived-macrophages (BMDMs)^[2] .
Ferulic acid methyl ester (25 μg/mL) inhibits COX-2 expression, blocks p-p38 and p-JNK^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:Primary bone marrow derived-macrophages (BMDMs)Concentration:25 μg/mLIncubation Time:6 h, 18 h, 48 hResult:Showed no cytotoxic effects on BMDMs.

208.21

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2309-07-1

阿魏酸甲酯

O=C(/C=C/C1=CC=C(C(OC)=C1)O)OC

Room temperature in continental US; may vary elsewhere.

Powder-20°C3 years4°C2 yearsIn solvent-80°C6 months-20°C1 month

In Vitro: 

DMSO : ≥ 100 mg/mL (480.28 mM)

H₂O : 2 mg/mL (9.61 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM4.8028 mL24.0142 mL48.0284 mL5 mM0.9606 mL4.8028 mL9.6057 mL10 mM0.4803 mL2.4014 mL4.8028 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式 选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  
  

  请依序添加每种溶剂： 10% DMSO    40% PEG300     5% Tween-80     45% saline

  Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

  
  

• 2.

  
  

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD  in saline)

  Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

  
  

• 3.

  
  

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

  
  

*以上所有助溶剂都可在 MCE 网站选购。

• [1]. Sultana R. Ferulic acid ethyl ester as a potential therapy in neurodegenerative disorders. Biochim Biophys Acta. 2012 May;1822(5):748-52.

  [2]. Phuong NT, et al. Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med. 2014 Sep;7S1:S327-31.