Rhodamine 800 has different optical absorption spectra in various organic solvents and in aqueous solution in the visible and near-infrared regions. The absorption maximum at 683 nm in acetone shifts to 696 nm in DMSO without a significant change of the overall spectral profile. The fluorescence intensity of Rhodamine 800 varies markedly with the solvent, and decreases to less than one-fifth when the solvent is changed from acetone to water without a significant change of the emission peak^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

495.95

C??H??ClN?O?

137993-41-0

罗丹明800

N#CC(C1=CC2=C3N(CCC2)CCCC3=C1[O+]=C45)=C4C=C6CCCN7CCCC5=C76.O=Cl(=O)([O-])=O

Room temperature in continental US; may vary elsewhere.

Powder-20°C3 years4°C2 yearsIn solvent-80°C6 months-20°C1 month

In Vitro: 

DMSO : 83.33 mg/mL (168.02 mM; Need ultrasonic)

Preparing
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM2.0163 mL10.0817 mL20.1633 mL5 mM0.4033 mL2.0163 mL4.0327 mL10 mM0.2016 mL1.0082 mL2.0163 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37.

  [2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49.