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DAF 2DA
  • 品牌:Chemstan
  • 产地:中国
  • 货号:CS-D0032
  • cas:205391-02-2
  • 价格: ¥500/支
  • 发布日期: 2020-11-25
  • 更新日期: 2025-09-22
产品详请
产地 中国
品牌 Chemstan
货号 CS-D0032
用途 标准品
包装规格
纯度 99%%
CAS编号 205391-02-2
是否进口

联合知名大学科研院所及企业开发药食两用植物标准品和天然植物有效单体,主打中药对照品/标准品/天然植物有效单体,小分子化合物库,药物杂质。所有产品仅用作科学研究,我们不为任何个人用途提供产品和服务。DAF 2DA 是测量 NO 最广泛使用的一种荧光探针。

Description

DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.

In Vitro

Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

446.41

Formula

C??H??N?O?

CAS No.

205391-02-2

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(N)C(N)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
  • [1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.


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