联合知名大学科研院所及企业开发药食两用植物标准品和天然植物有效单体，主打中药对照品/标准品/天然植物有效单体，小分子化合物库，药物杂质。所有产品仅用作科学研究，我们不为任何个人用途提供产品和服务。Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。Description

Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

In Vitro

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

580.65

Formula

C??H??N?NaO?S?

CAS No.

3520-42-1

中文名称

磺酰罗丹明 B；柴林红 B；奇通红 S；酸性桃红 B；酸性红 52；二甲苯红 B；酸性玫瑰红 B；磺化罗丹明 B；硫罗丹明 B；食品红 106

SMILES

O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro: 

DMSO : 50 mg/mL (86.11 mM; Need ultrasonic)

H₂O : 20 mg/mL (34.44 mM; Need ultrasonic)

Preparing 
Stock Solutions

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

References

• [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.