Two weeks after injection of stained cells, a single, bright, DiD perchlorate (DiD)-positive cells located between 15 to 40 μm from the endosteum is found. Results reveal a progressive appearance of cell clusters of decreased dye intensity, consistent with the partitioning of DiD perchlorate label on cell division^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

959.90

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127274-91-3

CCCCCCCCCCCCCCCCCCN1C2=C(C=CC=C2)C(C)(C)/C1=C\C=C\C=C\C3=[N+](C4=C(C=CC=C4)C3(C)C)CCCCCCCCCCCCCCCCCC.O=Cl(=O)([O-])=O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vitro: 

DMSO : 50 mg/mL (52.09 mM; Need ultrasonic)

Preparing 
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM1.0418 mL5.2089 mL10.4178 mL5 mM0.2084 mL1.0418 mL2.0836 mL10 mM0.1042 mL0.5209 mL1.0418 mL

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vivo:

请根据您的实验动物和给药方式 选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  
  

  请依序添加每种溶剂： 10% DMSO    40% PEG300     5% Tween-80     45% saline

  Solubility: ≥ 1.67 mg/mL (1.74 mM); Clear solution

  
  

• 2.

  
  

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD  in saline)

  Solubility: ≥ 1.67 mg/mL (1.74 mM); Clear solution

  
  

• 3.

  
  

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 1.67 mg/mL (1.74 mM); Clear solution

  
  

*以上所有助溶剂都可在 MCE 网站选购。

• [1]. Kenji Yumoto, et al. A novel method for monitoring tumor dormancy using fluorescent dye DiD. Cytometry A. 2014 Jun; 85(6): 548–555.

  [2]. Meng Li, et al. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J Vis Exp. 2017; (128): 56273.

  [3]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.