K114's unusually low buffer fluorescence is due to self-quenching in sedimentable aggregates or micelles which upon interacting with amyloid fibrils undergo an enhancement in fluorescence intensity and shifts in the excitation and emission spectra^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

393.27

C??H??BrO?

872201-12-2

BrC1=CC(/C=C/C2=CC=C(O)C=C2)=CC=C1/C=C/C3=CC=C(O)C=C3

Room temperature in continental US; may vary elsewhere.

Powder-20°C3 years4°C2 yearsIn solvent-80°C6 months-20°C1 month

In Vitro: 

DMSO : 100 mg/mL (254.28 mM; Need ultrasonic)

Preparing
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM2.5428 mL12.7139 mL25.4278 mL5 mM0.5086 mL2.5428 mL5.0856 mL10 mM0.2543 mL1.2714 mL2.5428 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. LeVine H 3rd, et al. Mechanism of A beta(1-40) fibril-induced fluorescence of (trans,trans)-1-bromo-2,5-bis(4-hydroxystyryl)benzene (K114). Biochemistry. 2005 Dec 6;44(48):15937-43.

  [2]. Selmani V, et al. K114 (trans, trans)-bromo-2,5-bis(4-hydroxystyryl)benzene is an efficient detector of cationic amyloid fibrils. Protein Sci. 2015 Mar;24(3):420-5.