Amyloid β-Peptide (1-42) (human) TFA
- 价格： ￥3500/支
- 发布日期： 2020-11-25
- 更新日期： 2025-09-22
产品详请
| 产地 |
中国
|
| 品牌 |
Chemstan
|
| 货号 |
CS-P1363
|
| 用途 |
分析检测
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| 包装规格 |
1mg
|
| 纯度 |
99%%
|
| CAS编号 |
107761-42-2
|
| 是否进口 |
否
|
Amyloid β-Peptide (1-42) human TFA 是由 42 个氨基酸组成的肽，其在阿尔茨海默病的发病机制中起关键作用。
| Description | β-Amyloid (1-42), human TFA (Amyloid β-Peptide (1-42) (human) TFA) is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer disease[1] . |
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| In Vitro | β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.
2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.
3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.
Methods vary depends on the downstream applications. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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| Molecular Weight | 4628.06 |
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| Formula | C???H???F?N??O??S |
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| CAS No. | 107761-42-2 |
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| Sequence | Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala |
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| Sequence Shortening | DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
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| Shipping | Room temperature in continental US; may vary elsewhere. |
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| Storage | Powder-80°C2 years-20°C1 yearIn solvent-80°C6 months-20°C1 month |
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| Solvent & Solubility | In Vitro: DMSO : 50 mg/mL (10.80 mM; Need ultrasonic) Preparing
Stock Solutions ConcentrationSolventMass1 mg5 mg10 mg1 mM0.2161 mL1.0804 mL2.1607 mL5 mM0.0432 mL0.2161 mL0.4321 mL10 mM0.0216 mL0.1080 mL0.2161 mL * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 |
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| References | [1]. Solntseva EI, et al. Impact of amyloid-β peptide (1-42) on voltage-gated ion currents in molluscan neurons. Bull Exp Biol Med. 2011 Oct;151(6):671-4. [2]. Barucker C, et al. Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription. J Biol Chem. 2014 Jul 18;289(29):20182-91. [3]. Stefania Sabella, et al. Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides. Electrophoresis. 2004 Oct;25(18-19):3186-94.
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