Polymyxin B nonapeptide, a cationic cyclic peptide derived by enzymatic processing from the naturally occurring peptide polymyxin B, is able to increase the permeability of the outer membrane of Gram-negative bacteria toward hydrophobic antibiotics probably by binding to the bacterial lipopolysaccharide (LPS)^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

963.13

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86408-36-8

Thr-{Dab}-{Dab}-{Dab}-{Dab}-d-Phe-Leu-{Dab}-{Dab}-Thr (Lactam: Dab3-Thr9)

T-{Dab}-{Dab}-{Dab}-{Dab}-d-FL-{Dab}-{Dab}-T (Lactam: Dab3-Thr9)

多粘菌素B九肽

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

In Vitro: 

H₂O : ≥ 100 mg/mL (103.83 mM)

DMSO : 16.67 mg/mL (17.31 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

Preparing 
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM1.0383 mL5.1914 mL10.3828 mL5 mM0.2077 mL1.0383 mL2.0766 mL10 mM0.1038 mL0.5191 mL1.0383 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式 选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  
  

  请依序添加每种溶剂： 10% DMSO    40% PEG300     5% Tween-80     45% saline

  Solubility: ≥ 1.67 mg/mL (1.73 mM); Clear solution

  
  

• 2.

  
  

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD  in saline)

  Solubility: ≥ 1.67 mg/mL (1.73 mM); Clear solution

  
  

• 3.

  
  

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 1.67 mg/mL (1.73 mM); Clear solution

  
  

*以上所有助溶剂都可在 MCE 网站选购。

• [1]. Tsubery H, et al. Structure-function studies of polymyxin B nonapeptide: implications to sensitization of gram-negative bacteria. J Med Chem. 2000 Aug 10;43(16):3085-92.

  [2]. Ofek I, et al. Antibacterial synergism of polymyxin B nonapeptide and hydrophobic antibiotics in experimental gram-negative infections in mice. Antimicrob Agents Chemother. 1994 Feb;38(2):374-7.