IC50: 9 μM (ACE)^[1] .

H-Val-Pro-Pro-OH (TFA), a proline peptides derivative, could inhibit Angiotensin I converting enzyme (ACE), with an IC₅₀ of 9 μM^[1] . H-Val-Pro-Pro-OH (TFA) could enhance insulin sensitivity and prevent insulin resistance in 3T3-F442A pre-adipocytes. H-Val-Pro-Pro-OH (TFA) also has anti-hypertensive and anti-inflammatory functions. H-Val-Pro-Pro-OH (TFA) further enhances the expression of glucose transporter 4 (GLUT4) in adipocytes and restores glucose uptake in TNF-treated adipocytes^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

425.40

C??H??F?N?O?

Room temperature in continental US; may vary elsewhere.

Powder-20°C3 yearsIn solvent-80°C6 months-20°C1 month

In Vitro: 

H₂O : 125 mg/mL (293.84 mM; Need ultrasonic)

Preparing
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM2.3507 mL11.7536 mL23.5073 mL5 mM0.4701 mL2.3507 mL4.7015 mL10 mM0.2351 mL1.1754 mL2.3507 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Nakamura Y, et al. Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk. J Dairy Sci. 1995 Apr;78(4):777-83.

  [2]. Chakrabarti S, et al. Milk-Derived Tripeptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) Enhance Insulin Sensitivity and Prevent Insulin Resistance in 3T3-F442A Preadipocytes. J Agric Food Chem. 2018 Oct 3;66(39):10179-10187.