Amyloid-β^[1]

Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC₅₀ of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC₅₀) is examined. The IC₅₀ is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

597.41

C??H??Cl?N?

CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(N(C)C)C=C6)N5)=C4)N3)=C2)CC1.[H]Cl.[H]Cl.[H]Cl.[H]Cl

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

In Vitro: 

H₂O : 2.67 mg/mL (4.47 mM; Need ultrasonic)

Preparing
Stock Solutions

ConcentrationSolventMass1 mg5 mg10 mg1 mM1.6739 mL8.3695 mL16.7389 mL5 mM---------10 mM---------

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Thai NQ, et al. Discovery of DNA dyes Hoechst 34580 and 33342 as good candidates for inhibiting amyloid beta formation: in silico and in vitro study. J Comput Aided Mol Des. 2016 Aug;30(8):639-50.