In the presence of Transdermal Peptide Disulfide, because of the specific binding of Transdermal Peptide Disulfide to ATP1B1, cells will upregulate the level of ATP1B1 to maintain function and structure; as a result, the expression of ATP1B1 increases. However, as time goes on, some Transdermal Peptide Disulfide molecules may be transported into cells by endocytosis; consequently, the expression of ATP1B1 then decreases. The interaction between Transdermal Peptide Disulfide and ATP1B1 changes not only the expression of ATP1B1, but also the localization of ATP1B1 and then the structure of the epidermal layer. This interaction can be attenuated by inhibitors or competitors, which would result in the reduced delivery of macromolecular drugs across the skin^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1061.15

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888486-23-5

Ala-Cys-Ser-Ser-Ser-Pro-Ser-Lys-His-Cys-Gly (Disulfide bridge:Cys2-Cys10)

ACSSSPSKHCG (Disulfide bridge:Cys2-Cys10)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

In Vitro: 

H₂O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign valueAcidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetailsNegative (<0)1.  Try to dissolve the peptide in water first.2.  If water fails, add NH4OH (<50 μL).3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.Positive (>0)1.  Try to dissolve the peptide in water first.2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

• [1]. Wang C, et al. Role of the Na(+)/K(+)-ATPase beta-subunit in peptide-mediated transdermal drug delivery. Mol Pharm. 2015 Apr 6;12(4):1259-67.