Ki: 0.21 nM (human MC1 receptor)^[1]

ACTH (1-17) TFA is a potent agonist at the hMC1R. ACTH (1-17) shows high affinity for the hMC1R with a K_i value of 0.21±0.03 nM which is slightly higher than that of 0.13±0.005 nM for alpha-MSH^[1] . ACTH (1-17) induces a slight and not significant increase in growth hormone secretion even when micromolar concentrations of the peptide are employed in rat pituitary cultures^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Inhibition of DNA labeling is noted when the ACTH (1-17) is administered at 2 hr after the beginning of the daily dark span when nocturnal animals become active. When administered at this circadian stage, the larger dose in particular is associated with an inhibition of DNA labeling lasting for 24 hr. The inhibitory effect is much shorter when the same dose is injected 4 hr earlier^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

2207.43

C??H???N??O??S.C?HF?O?

Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg

SYSMEHFRWGKPVGKKR

Room temperature in continental US; may vary elsewhere.

Protect from light

Powder-80°C2 years-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

In Vitro: 

H₂O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign valueAcidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetailsNegative (<0)1.  Try to dissolve the peptide in water first.2.  If water fails, add NH4OH (<50 μL).3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.Positive (>0)1.  Try to dissolve the peptide in water first.2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

• [1]. Tsatmali M et al. ACTH1-17 is a more potent agonist at the human MC1 receptor than alpha-MSH. Cell Mol Biol (Noisy-le-grand). 1999 Nov;45(7):1029-34.

  [2]. Ceda GP, et al. The effects of ACTH (1-17) on GH secretion in vitro. Horm Metab Res. 1987 Aug;19(8):361-3.

  [3]. Walker WV, et al. Effect of an adrenocorticotropin analogue, ACTH (1-17), on DNA synthesis in murine metaphyseal bone. Biochem Pharmacol. 1985 Apr 15;34(8):1191-6.