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α-Factor Mating Pheromone, yeast (TFA)
  • 品牌:Chemstan
  • 产地:中国
  • 货号:HY-P1482A
  • 价格: ¥800/支
  • 发布日期: 2020-11-24
  • 更新日期: 2025-09-19
产品详请
产地 中国
品牌 Chemstan
货号 HY-P1482A
用途 分析检测
包装规格 1mg
纯度 99%%
CAS编号
是否进口

α-Factor Mating Pheromone, yeast (TFA) 是一种十三肽,由?S. cerevisiae?的 α 细胞分泌产生,通过?Ste2p?受体起作用。

Description

α-Factor Mating Pheromone, yeast (TFA) is a tridecapeptide secreted by S. cerevisiae α cells via Ste2p receptor[1] .

Molecular Weight

1798.02

Formula

C??H???N??F?O??S

Sequence

Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr

Sequence Shortening

WHWLQLKPGQPMY

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Protect from light

Powder-80°C2 years-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign valueAcidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetailsNegative (<0)1.  Try to dissolve the peptide in water first.2.  If water fails, add NH4OH (<50 μL).3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.Positive (>0)1.  Try to dissolve the peptide in water first.2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

References
  • [1]. [1]. Naider F, et al. The alpha-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep;25(9):1441-63.


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