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| 产地 | 中国 |
| 品牌 | Chemstan |
| 货号 | HY-P1883 |
| 用途 | 分析检测 |
| 包装规格 | 1mg |
| 纯度 | 99%% |
| CAS编号 | |
| 是否进口 | 否 |
Bacterial Sortase Substrate III, Abz/DNP 是一种内部淬灭的荧光肽底物。金黄色葡萄球菌转肽酶分选酶A (SrtA) 与 Bacterial Sortase Substrate III, Abz/DNP 反应，裂解并催化苏氨酸的羧基与细胞壁交联桥的氨基之间形成酰胺键。可以在 Ex/Em=320 nm/420 nm 下检测该底物的切割。
| Description | Bacterial Sortase Substrate III, Abz/DNP TFA is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm. |
|---|---|
| Molecular Weight | 1042.02 |
| Formula | C??H??N??F?O?? |
| Sequence | Abz-Leu-Pro-Glu-Thr-Gly-{Lys(Dnp)}-NH2 |
| Sequence Shortening | Abz-LPETG-K(Dnp)-NH2 |
| Shipping | Room temperature in continental US; may vary elsewhere. |
| Storage | Powder-80°C2 years-20°C1 yearIn solvent-80°C6 months-20°C1 month |
| Solvent & Solubility | In Vitro: H2O Peptide Solubility and Storage Guidelines: 1. Calculate the length of the peptide. 2. Calculate the overall charge of the entire peptide according to the following table: ContentsAssign valueAcidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0 3. Recommended solution: Overall charge of peptideDetailsNegative (<0)1. Try to dissolve the peptide in water first.2. If water fails, add NH4OH (<50 μL).3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.Positive (>0)1. Try to dissolve the peptide in water first.2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.Zero (=0)1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
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