概述（Summary）icon

英文全称

Recombinant Human VIL1 protein

纯度（Purity）

>90% as determined by SDS-PAGE

内毒素（Endotoxin level）

Please contact with the lab for this information.

蛋白构建（Construction）

A DNA sequence encoding the human VIL1 (Met1-Lys320) was fused with His tag

Accession #

P09327

表达宿主（Host）

E.coli

种属（Species）

Homo sapiens (Human)

预测分子量（Predicted Molecular Mass）

35.98 kDa

制剂（Formulation）

Supplied as solution form  in  PBS pH 7.5./ Lyophilized from PBS pH 7.5.

运输方式（Shipping）

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

稳定性&储存（Stability &Storage）

Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.

复溶（Reconstitution）

Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.

背景（Background）icon

背景介绍

Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

分子别名（Alternative Names）

Villin-1.