产品名（Product Name）icon

Anti Extracellular Death Factor mouse monoclonal antibody

货号（Catalog No.）icon

CS10233Mo

种类（Category）icon

Primary antibody

宿主（Host）icon

Mouse

反应种属（Species specificity）icon

Escherichia coli

应用实验（Tested applications）icon

Elisa,IHC

克隆性（Clonality）icon

Monoclonal

克隆编号（Clone No.）icon

93-D-4

偶连物（Conjugation）icon

Unconjugate

免疫原（Immunogen）icon

NNWNN

别名icon

Description: mazEF is a toxin-antitoxin stress-induced module which is abundant on the chromosome of most bacteria including pathogens and most extensively studied in Escherichia coli. E. coli mazEF mediated cell death is a population phenomenon requiring the quorum-sensing (QS) ‘Extracellular Death Factor’ (EDF), the E. coli peptide NNWNN. Penta-peptide NNWNN (EcEDF) enhances the endoribonucleolytic activity of E. coli toxin MazF.The newly discovered extracellular death factor (EDF) is a pentapeptide with the sequence NNWNN in Escherichia coli. It was reported that it participated in the cell death process mediated by toxin-antitoxin system mazEF. Reactive oxygen species (ROS) are recently considered as common factors for bactericidal antibiotics-mediated cell death. Previous study indicated that EDF could scavenge hydroxyl radicals and might act as a signal molecule with dual effects, “death” and “survival”. But the structure-activity relationship of EDF and the effects of EDF on the activity of antibiotics remain unclear. In the present study, our results indicated that tryptophan could be the key residue to the hydroxyl radicals-scavenging activity of EDF, and EDF could protect Escherichia coli from killing by bactericidal antibiotics, but not by DNA-damaging or bacteriostatic antibiotics. Our results could provide novel evidence to understand the role of EDF in drug-resistance.

Uniprot IDicon

P0AC53

状态（Form）icon

Liquid

储存溶液（Buffer）icon

PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.

存放条件（Storage）icon

Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 4 °C for frequent use.
Store at -20 to -80 °C for twelve months from the date of receipt.

浓度（Concentration）icon

1mg/ml

亚型（Isotype）icon

IgG2b

分子量（MW）icon

56 kDa

纯化方式（Purity）icon

Protein G purified from mice ascites