(rel)-Asperparaline A ((rel)-Aspergillimide), an anthelmintic metabolite, is isolated from okara that has been fermented with Aspergillus japonicas JV-23. (rel)-Asperparaline A is also a potent and selective antagonist of nAChR. (rel)-Asperparaline A exhibits paralytic activity in silk worms^{[1] [2]} .

Asperparaline A (1 μM; 1 min) markedly and reversibly blocks the acetylcholine (Ach; 10 μM)-induced current in the silkworm larval neurons^[2] .
Asperparaline A (1-1000 nM; 1 min) differentially blocks the peak and slowly desensitizing currents, with IC₅₀s of 20.2 and?39.6 nM, respectively^[2] .
Asperparaline A (10 μM; 1 min) reduces the peak current amplitude of the ACh (100 μM)-induced response of α3β4 nAChR by 33.4%, while barely influencing the amplitudes of the responses to ACh of the α4β2 and α7 nAChRs in X. laevis oocytes^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Asperparaline A (10 μg/g; p.o.) exhibits paralysis against silk worms within 1 h and lasts for 7 to 10 h^[1] .
Asperparaline A (3 μg/g; injection with a micro-syringe) exhibits paralysis activity against silk worms within 20 min and lasts for 4 to 5 h^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

359.46

C??H??N?O?

195966-93-9

CN(C1=O)C(C[C@@]2(CC3=O)C(N3C)=O)(CN(CC[C@@H]4C)C14C5)[C@]5([H])C2(C)C.[Rotation (-)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hayashi H, et, al. New paralytic alkaloids, asperparalines A, B and C, from Aspergillus japonicus JV-23. Biosci Biotechnol Biochem. 2000 Jan;64(1):111-5.

  [2]. Hirata K, et, al. A fungal metabolite asperparaline a strongly and selectively blocks insect nicotinic acetylcholine receptors: the first report on the mode of action. PLoS One. 2011 Apr 1;6(4):e18354.