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Description
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Splenopentin diacetate is a synthetic immunomodulating pentapeptide corresponding to the residues 32-36 of the splenic hormone splenin. Splenopentin diacetate influences both early T and B cell differentiation, to increase the number of antibody-forming cells in mice after gamma irradiation[1] [2] .
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In Vitro
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Splenopentin (1-100 μM) augments human NK cells and has no cytotoxicity[2] .
Splenopentin (0.1 ng/mL-10 μg/mL; 3 h) induces phenotypic differentiation of both T- and B-cell precursor cells[3] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
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In Vivo
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Splenopentin (1 mg/kg; i.p. three times a week for 7-90 d) accelerates Langerhans cell recruitment and leads to pretreatment levels of Langerhans cell (LC) density in the skin of mice[4] .
Splenopentin (0.01-100 μg per mouse; i.p.) has no demonstrable effect on neuromuscular transmission of mice[3] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:
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Female Balb/c/Bln mice were injected Cyclophosphamide or Dexamethasone for 5 consecutive days[4]
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Dosage:
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1 mg/kg
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Administration:
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I.p. three times a week for 7-90 days
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Result:
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Reached the normal number of epidermal LCs at 28 days after the beginning of Cyclophosphamide treatment.
Accelerated restoration process and reached the normal LC density in the skin after 70 days in Dexamethasone treatment grops.
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Molecular Weight
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Formula
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CAS No.
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中文名称
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Shipping
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Room temperature in continental US; may vary elsewhere.
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Storage
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Please store the product under the recommended conditions in the Certificate of Analysis.
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Solvent & Solubility
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In Vitro:
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
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Contents
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Assign value
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Acidic amino acid
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Asp (D), Glu (E), and the C-terminal -COOH.
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-1
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Basic amino acid
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Arg (R), Lys (K), His (H), and the N-terminal -NH2
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+1
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Neutral amino acid
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Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
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0
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3. Recommended solution:
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Overall charge of peptide
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Details
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Negative (<0)
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1. Try to dissolve the peptide in water first.
2. If water fails, add NH4OH (<50 μL).
3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
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Positive (>0)
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1. Try to dissolve the peptide in water first.
2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
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Zero (=0)
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1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
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References
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[1]. Singh VK, et, al. Thymopentin and splenopentin as immunomodulators. Current status. Immunol Res. 1998;17(3):345-68.
[2]. Rastogi A, et, al. Augmentation of human natural killer cells by splenopentin analogs. FEBS Lett. 1993 Feb 8;317(1-2):93-5.
[3]. Audhya T, et, al. Contrasting biological activities of thymopoietin and splenin, two closely related polypeptide products of thymus and spleen. Proc Natl Acad Sci U S A. 1984 May;81(9):2847-9.
[4]. Gruner S, et, al. Stimulation of the recruitment of epidermal Langerhans cells by splenopentin. Arch Dermatol Res. 1990;281(8):526-9.
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