产品名称：5-IAF 5-(碘乙酰胺基)荧光素

CAS：63368-54-7

规格：25mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：515.25

溶剂：DMSO

激发波长（nm）：497

发射波长（nm）：516

产品介绍

5-IAF 5-(碘乙酰胺基)荧光素是美国AAT Bioquest生产的荧光素 。5-IAF与马来酰亚胺荧光素一样是用于巯基改性蛋白最普遍的绿色荧光染料。

参考文献

Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015

Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75

Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726

Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726

Distances between 5-iodoacetamidofluorescein and the ATP and ouabain sites of (Na,K)-ATPase determined by fluorescence energy transfer
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197

5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2090

Ligand binding to (Na,K)-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2084

Steady-state fluorescence emission from the fluorescent probe, 5-iodoacetamidofluorescein, bound to hemoglobin
Authors: Hirsch RE, Zukin RS, Nagel RL.
Journal: Biochem Biophys Res Commun (1986): 489

Fluorescent labeling of (Na+ + K+)-ATPase by 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: Biochim Biophys Acta (1982): 493

Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin
Authors: Wang YL, Taylor DL.
Journal: J Histochem Cytochem (1980): 1198