产品名称：生物素-4-荧光素 

CAS：1032732-74-3

规格：5mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：644.69

溶剂：DMSO

激发波长（nm）：492

发射波长（nm）：518

产品介绍

生物素-4-荧光素是美国AAT Bioquest生产的荧光染料 。这种具有两种功能的生物素偶联物比生物素、荧光素具有更强的结合能力和更强的荧光。从高结合能力、快速结合、不与亲和素和链霉亲和素四聚体协同结合这几方面来讲，它具有与类似亲和素的结合特性， 这些所期待的特性可以把它归类于小尺寸/长度的新配体。因为大/长的生物素衍生物都存在特征性的彼此空间阻碍和在亲和素和链霉亲和素四聚体比例为4:1的混合物中有抗协同结合作用。生物素-荧光素这种特殊的结合相对于亲和素和链霉亲和素，伴随着84%~88%的配体荧光淬灭。它用来定量生物素结合位点。四个生物结合位点，链霉亲和素、亲和素、荧光染料的链霉亲和素偶联物或者酶中的任何一个被结合，发射荧光和光吸收都会淬灭。 因此，知道生物素-4-荧光素的浓度和链霉亲和素的量，就可以估计生物素结合位点的数量。

参考文献

Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin
Authors: Wu Y, Simons PC, Lopez GP, Sklar LA, Buranda T.
Journal: Anal Biochem (2005): 221

Electrophoretic behavior of streptavidin complexed to a biotinylated probe: a functional screening assay for biotin-binding proteins
Authors: Humbert N, Zocchi A, Ward TR.
Journal: Electrophoresis (2005): 47

Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein
Authors: Aslan FM, Yu Y, Mohr SC, Cantor CR.
Journal: Proc Natl Acad Sci U S A (2005): 8507

Preparation and characterisation of antibody modified gelatin nanoparticles as drug carrier system for uptake in lymphocytes
Authors: Balthasar S, Michaelis K, Dinauer N, von Briesen H, Kreuter J, Langer K.
Journal: Biomaterials (2005): 2723

Controlled surface functionalization of silica nanospheres by covalent conjugation reactions and preparation of high density streptavidin nanoparticles
Authors: Schiestel T, Brunner H, Tovar GE.
Journal: J Nanosci Nanotechnol (2004): 504

Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription
Authors: Huang F, Wang G, Coleman T, Li N.
Journal: Rna (2003): 1562

A novel intravascular drug delivery method using endothelial biotinylation and avidin-biotin binding
Authors: Hoya K, Guterman LR, Miskolczi L, Hopkins LN.
Journal: Drug Deliv (2001): 215

Organelle pH studies using targeted avidin and fluorescein-biotin
Authors: Wu MM, Llopis J, Adams S, McCaffery JM, Kulomaa MS, Machen TE, Moore HP, Tsien RY.
Journal: Chem Biol (2000): 197

Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate
Authors: Kada G, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 33

Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization
Authors: Kada G, Kaiser K, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 44