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即用型ABTS溶液 适合HRP标记的ELISA检测
  • 品牌:AAT Bioquest
  • 产地:American
  • 型号:1 L
  • 货号:CS11001
  • cas:30931-67-0
  • 价格: ¥2328/支
  • 发布日期: 2022-03-01
  • 更新日期: 2025-09-19
产品详请
产地 American
品牌 AAT Bioquest
货号 CS11001
用途 Research Grade
包装规格 1 L
纯度 98%%
CAS编号 30931-67-0
是否进口
简要概述

即用型ABTS溶液是美国AAT Bioquest生产的ELISA检测试剂 ,辣根过氧化物酶(HRP)和HRP缀合物在过氧化氢存在下促进ABTS氧化,将ABTS转化为其蓝绿色氧化产物。该显色反应广泛用于ELISA测定中的HRP定量。氧化的ABTS产物具有420nm的最大吸收,可以用分光光度计容易地跟踪。ReadiUse™ABTS解决方案针对在微孔板或试管中使用HRP或HRP标记的缀合物和过氧化氢的ELISA测定进行了优化。我们的ABTS解决方案可以在一次添加中完成HRP反应。在过氧化氢存在下,当与HRP或HRP缀合物反应时,测定溶液将其颜色改变为浅绿色。


适用仪器


光吸收酶标仪
吸收: 420nm
推荐孔板: 纯白色


产品说明书

样品实验方案

1.在HRP标记的试剂温育后洗涤测定板。
2.每孔加入100μLReadiUse ABTS溶液。
3.将板在室温下孵育15-30分钟。注意:孵育时间取决于测定条件。
4.用ELISA酶标仪测量415±10 nm(最大420 nm)的吸光度信号。注意:如果需要,可以通过向0.1M柠檬酸中加入等体积的1%SDS或0.01%叠氮化钠来终止反应。
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