样品实验方案

以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。

溶液配制

储备溶液配制

操作步骤

1. 根据需要处理样品以诱导凋亡。
2. 添加Z-LETD-FMK。
   注意：我们建议使用最终浓度为10-100 μM的Z-LETD-FMK抑制胱天蛋白酶，例如对caspase-8抑制50 μM。
   注意：孵育的最佳时间和浓度需要通过实验确定。
3. 根据需要对样品染色。
4. 用适当的仪器检测荧光强度。 

图示

图1.用caspase 8抑制剂Z-LETD-FMK（Cat＃13307）抑制Jurkat细胞中caspase 8活性。当天，将Jurkat细胞以200,000个细胞/ 90 μL /孔的密度接种在Costar黑色96孔板中。用1 μM星形孢菌素处理细胞5小时。加入Z-LETD-FMK并与星形孢菌素共孵育。用Cell Meter Caspase 8活性细胞凋亡检测试剂盒（Cat#22816 ）检测caspase-8活性。用Flexstation 3荧光酶标仪（Molecular Devices）在Ex / Em ＝ 540 / 620nm（截止＝ 610nm）下测量荧光强度。

参考文献

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Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury.
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Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells.
Authors: Ji, C and Ren, F and Xu, M
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[Synergistic effects and mechanisms of combined tumor necrosis factor-related apoptosis-inducing ligand and chemotherapeutic drugs or radiotherapy in killing laryngeal squamous carcinoma cells in vitro].
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Emodin azide methyl anthraquinone derivative triggers mitochondrial-dependent cell apoptosis involving in caspase-8-mediated Bid cleavage.
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Caspase-9 takes part in programmed cell death in developing mouse kidney.
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