产品名称：罗丹明123 

CAS：62669-70-9

规格：25mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：380.82

溶剂：DMSO

激发波长（nm）：507

发射波长（nm）：529

产品介绍

罗丹明123是美国AAT Bioquest生产的罗丹明染料 。带正电荷的罗丹明染料（如罗丹明酯和罗丹明）选择性地定位于线粒体中，因此它们广泛用于标记活细胞的线粒体。其中罗丹明123是最常用的线粒体染色剂。它用于染色各种细胞，包括植物细胞和细菌。与JC-1一样，罗丹明123除了用于选择性线粒体染色外，还用于测量线粒体膜电位。由于罗丹明123选择性地定位于癌细胞，因此可以使用罗丹明123来检测癌细胞，并且其荧光与线粒体分布的改变密切相关。罗丹明123通常用于流式细胞术中作为Pgp的功能性报道分子。最近的报道表明罗丹明123也可以是MRP1的底物。MDR蛋白的功能测定是癌症治疗中比MDR蛋白表达水平更好的预后指标。与罗丹明123类似的罗丹明123的光谱特性使得罗丹明123的使用非常方便，因为几乎所有荧光仪器都配备有激发源和FITC滤光片组。罗丹明123可用于多参数分析，无需荧光干扰，与常见的蛋白质标记染料如PE-Cy5和AMCA结合使用。

参考文献

Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779

Rhodamine 123 permeability through the catfish intestinal wall: Relationship to thermal acclimation and acute temperature change
Authors: Kleinow KM, Johnston BD, Holmes EP, McCarrol ME.
Journal: Comp Biochem Physiol C Toxicol Pharmacol (2006): 205

[Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection]
Authors: Song JP.
Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban (2006): 610

Alterations in respiration rate of isolated rainbow trout hepatocytes exposed to the P-glycoprotein substrate rhodamine 123
Authors: Bains OS, Kennedy CJ.
Journal: Toxicology (2005): 87

Assessment of drug interactions in hepatobiliary transport using rhodamine 123 in sandwich-cultured rat hepatocytes
Authors: Annaert PP, Brouwer KL.
Journal: Drug Metab Dispos (2005): 388

Determination of Rhodamine 123 in cell lysate by HPLC with visible wavelength detection
Authors: Iqbal T, Kinjo M, Dowling TC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 259

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767

Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430

Rhodamine-123: therapy for hormone refractory prostate cancer, a phase I clinical trial
Authors: Jones LW, Narayan KS, Shapiro CE, Sweatman TW.
Journal: J Chemother (2005): 435