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| 产地 | American |
| 品牌 | AAT Bioquest |
| 货号 | CS22784 |
| 用途 | Research Grade |
| 包装规格 | 1 kit |
| 纯度 | 98%% |
| CAS编号 | |
| 是否进口 | 是 |
该试剂盒有多种参数可用于监控细胞活力 。试剂盒中使用的专有的紫色激光激发荧光染料是一种疏水性化合物，可轻松渗透完整的活细胞，并在进入活细胞后获得增强的荧光。细胞内酯酶对非荧光底物的水解产生了强烈的蓝色荧光产物，该产物很好地保留在细胞质中。通过非荧光底物的酯酶水解产生的蓝色荧光团具有荧光素的光谱特性。当在405 nm激发时，荧光团在?450 nm处发出强烈的蓝色荧光。该试剂盒提供了所有必需成分，并具有用于荧光微孔板检测的优化的细胞标记方案。此Cell Meter 细胞活力分析试剂盒为荧光流式细胞术，微孔板和细胞功能的显微镜研究提供了标记细胞的有效工具。它可用于多种研究，包括细胞粘附，趋化性，多药耐药性，细胞生存力，细胞凋亡和细胞毒性。该试剂盒适用于增殖和非增殖细胞。
适用仪器
| 光吸收酶标仪 | |
| 激发： | 405nm |
| 发射： | 460nm |
| cutoff： | 435nm |
| 推荐孔板： | 黑色透明 |
| 读取模式： | 底读模式 |
样品实验方案
简要概述
溶液配制
储备溶液配制
CytoCalcein™Violet 450，AM储备溶液：
将20 μL DMSO（组分B）添加到CytoCalcein™Violet 450，AM（组分A）的小瓶中，并充分混合以制成CytoCalcein™Violet 450，AM储备溶液。注意：20 μL CytoCalcein™Violet 450，AM储备液足以装满一块板。避光。为了存放，请将管子紧紧密封。
工作溶液配制
将CytoCalcein™Violet 450，AM储备溶液的全部内容物（20 μL）加入10 mL的测定缓冲液（组分C）中，并充分混合以制成CytoCalcein™Violet 450，AM工作溶液。该CytoCalcein™Violet 450，AM工作溶液在室温下至少可稳定2小时。注意：如果诸如CHO细胞之类的细胞含有能促进荧光染料随时间渗漏的有机阴离子转运蛋白，则应制备丙磺舒储备溶液并以最终孔内工作浓度范围为1至1的浓度添加到上样缓冲液中。 2.5毫米 分装并保存未使用的丙磺舒储备溶液于≤-20°C。
操作步骤
参考文献
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