产品名称：活细胞荧光示踪探针CDCFDA

规格：100mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：529.28

溶剂：DMSO

激发波长（nm）：504

发射波长（nm）：529

产品介绍

活细胞荧光示踪探针CDCFDA是美国AAT Bioquest生产的用于开发荧光生物共轭物的荧光染料 。众所周知，细胞的荧光标记是确定总细胞数或样品中存在多少活细胞的有效方法。流式细胞仪结合荧光染色是分析异质细胞群的有力工具。荧光素二乙酸酯（FDA）及其衍生物是非荧光分子，其扩散到细胞中并被细胞内非特异性酯酶水解以产生荧光产物。荧光产物只能在具有完整细胞膜的细胞中积累; 因此，具有渗漏膜的死细胞不会被染色。FDA及其类似物（如CDCFDA）的膜转运和细胞内水解的精确动力学与细胞功能有关，因此FDA标记可用于通过流式细胞术或荧光显微镜监测细胞。FDA染料标记细胞的荧光强度在细胞系和菌株之间差异很大，可能是由于细胞内酯酶活性的差异。

参考文献

Cationic block amphiphiles show anti-mitochondrial activity in multi-drug resistant breast cancer cells
Authors: Petro P Czupiel, Vianney Delplace, Molly S Shoichet
Journal: Journal of Controlled Release (2019)

Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
Authors: Yufan Xu, Xiaohong Wang
Journal: Biotechnology and bioengineering (2015): 1683--1695

Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses
Authors: Yan-Xu Yin, Shu-Bin Wang, Huai-Juan Xiao, Huai-Xia Zhang, Zhen Zhang, Hua Jing, Ying-Li Zhang, Ru-Gang Chen, Zhen-Hui Gong
Journal: International journal of molecular sciences (2014): 20101--20116

Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay
Authors: Harold Obiakor, Marion Avril, Nicholas J MacDonald, Prakash Srinivasan, Karine Reiter, Charles Anderson, Kevin L Holmes, Michal Fried, Patrick E Duffy, Joseph D Smith
Journal: Clinical and Vaccine Immunology (2013): 433--442

Aging enhances maceration-induced ultrastructural alteration of the epidermis and impairment of skin barrier function
Authors: Takeo Minematsu, Yuko Yamamoto, Takashi Nagase, Ayumi Naito, Kimie Takehara, Shinji Iizaka, Kazunori Komagata, Lijuan Huang, Gojiro Nakagami, Tomoko Akase
Journal: Journal of dermatological science (2011): 160--168