产品名称：新型钙离子荧光探针Calbryte 630,钾盐

规格：5x50 ug

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1091.06

溶剂：水

激发波长（nm）：608

发射波长（nm）：626

产品介绍

新型钙离子荧光探针Calbryte 630,钾盐是美国AAT Bioquest生产的钙离子荧光探针 ，钙测量对于许多生物学研究至关重要。显示结合钙后光谱响应的荧光探针使研究人员能够使用荧光显微镜，流式细胞仪，荧光光谱和荧光酶标仪来研究细胞内游离钙浓度的变化。x-Rhod-1通常用作红色荧光钙指示剂。但是，x-Rhod-1在酯酶水解后在活细胞中仅适度发荧光，并且细胞钙反应非常小。Calbryte 630的开发旨在改善x-Rhod-1的细胞负载和钙反应，同时保持x-Rhod-1的光谱波长，使其与TexasRed®滤光片兼容。在CHO和HEK细胞中，Cal-630 AM的细胞钙反应比x-Rhod-1敏感得多。Calbryte 630的光谱与FITC，AlexaFluor®488和GFP的光谱完全分离，使其成为与GFP细胞系或FITC / AlexaFluor®488标记的抗体进行细胞内分析多重检测的理想钙探针。Calbryte 630是用于测量细胞内钙的新一代红色荧光指示剂。Calbryte 630 AM的信号/背景比和细胞内保留特性大大提高，使其成为评估GPCR和钙通道靶标以及在活细胞中筛选其激动剂和拮抗剂的最强大的深红色荧光指示剂。像其他染料AM细胞上样一样，Calbryte 630 AM酯是非荧光的，一旦进入细胞内部，它就会被细胞内酯酶水解并被激活。

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