产品名称：ReadiUse 哺乳动物细胞裂解液*5X*

规格：10ml

储存条件：保存在冰箱2-8℃干燥

保质期：6个月

样品分析方案

1.在使用前在室温下使用ReadiUse 哺乳动物细胞裂解缓冲液* 5X *。

2.通过将1ml 5X Screen ReadiUse 哺乳动物细胞裂解缓冲液加入4 ml Mili-Q水中，制备1X ReadiUse™哺乳动物细胞裂解缓冲液，并充分混合。

3.现在可以使用1X ReadiUse 哺乳动物细胞裂解缓冲液（来自步骤2）。

4.根据需要处理细胞，用PBS洗涤板以除去残留的培养基。

5.说明书中的表是裂解贴壁细胞的建议体积。

6.通过将约100μL的1X ReadiUse 哺乳动物细胞裂解缓冲液加入到10⁶细胞沉淀中，得到非贴壁细胞。

7.在室温下孵育10-20分钟。

8.细胞裂解液已准备好进行下一步的生化分析。

参考文献

Rhinacanthin C Alleviates Amyloid-β Fibrils’ Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Kai-An Chuang, Ming-Han Li, Ni-Hsuan Lin, Chih-Hsuan Chang, I Lu, I Pan, Tomoya Takahashi, Ming-Der Perng, Shu-Fang Wen
Journal: Oxidative Medicine and Cellular Longevity (2017)

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Xiaoying Zhang, Cuicui Zhu, Qiong Luo, Jv Dong, Lv Liu, Min Li, Hongtao Zhu, Xiangping Ma, Jun Wang
Journal: Molecular Medicine Reports (2016): 3595--3601

Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Patrick Scheiermann, Malte Bachmann, Itamar Goren, Bernhard Zwissler, Josef Pfeilschifter, Heiko Mühl
Journal: The American journal of pathology (2013): 1107--1113

Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Dhaval Patel, Susan E Vandromme, Michael E Reid, Lakeshia J Taite
Journal: Biomacromolecules (2012): 1420--1428