具有DAPI的FluoroQuest 是一种水性固定介质，用于保留组织和细胞涂片的荧光。这种封固剂含有抗褪色剂以降低染料的光漂白速率，并以DAPI强化，DAPI是DNA的复染剂。用于原位杂交技术或需要荧光法进行DNA染色的其他方法。DAPI在360 nm处激发并在460 nm处发射，产生蓝色荧光。

样品实验方案

以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。

1. 预热溶液：从冰箱中取出安装溶液。将瓶子加热至室温，避光。
2. 添加溶液：清除样品中多余的液体。向样品中加一滴固定溶液。如果样品在载玻片或组织培养皿上，请小心地将盖玻片放在液滴上，以免产生气泡。如果样品在盖玻片上，请将盖玻片倒在干净的玻璃载玻片上。去除多余的防褪色成分。
3. 准备用于成像的样品：固定溶液应孵育2小时至过夜。要长期存放，请用指甲油或塑料密封剂将盖玻片密封在玻片上。已安装的载玻片应在黑暗中于4°C存放，荧光成像将保持稳定数周，样品可以在安装后立即成像。 

图示

图1. 甲醛固定，石蜡包埋的人肺腺癌阳性组织的荧光IHC 。用兔抗EpCam抗体对人肺腺癌阳性组织切片进行染色，然后分别与iFluor 488 Styramide （Cat＃CS45020 ）染色的聚HRP标记的羊抗兔IgG二抗一起孵育。将组织固定在带有DAPI（Cat#CS20005）的FluoroQuest 抗褪色固定介质中。

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