|
| 产地 | American |
| 品牌 | AAT Bioquest |
| 货号 | CS17665 |
| 用途 | Research Grade |
| 包装规格 | 100 Tests |
| 纯度 | 98%% |
| CAS编号 | |
| 是否进口 | 是 |
在进行各种分析的DNA样品制备中，DNA定量是一项非常重要的任务。 Portelite 荧光DNA定量试剂盒提供了使用Qubit荧光仪操作Helixyte Green BR快速定量dsDNA的方法。它针对Cytocite 和Qubit 荧光仪进行了优化。 Portelite 荧光DNA定量测定在三个数量级上呈线性。该测定法对RNA上的双链DNA（dsDNA）具有高度选择性，并针对测量10 pg / μL至10 ng / μL的dsDNA浓度进行了优化。Helixyte Green-BR与dsDNA结合后表现出较大的荧光增强，并且比UV吸光度读数高几个数量级。
适用仪器
| Qubit 荧光计 | |
| 激发： | 480nm |
| 发射： | 530nm |
| 器材： | 0.2 mL PCR小瓶 |
| CytoCite 荧光计 | |
| 激发： | 480nm |
| 发射： | 530nm |
| 器材： | 0.2 mL PCR小瓶 |
样品实验方案
简要概述
注意：打开之前，请将所有成分置于室温下。
溶液配制
工作溶液配制
Helixyte Green-BR工作溶液：在DNA分析缓冲液（组分B）中稀释200倍Portelite dsDNA试剂（组分A）。 例如，要为8个样品准备足够的工作溶液，请将5uL Helixyte Green BR（组分A）添加到1mL DNA分析缓冲液（组分B）中。注意：通过用箔纸覆盖或将其置于黑暗中来保护工作液免受光照。 我们建议在塑料容器中操作而不是玻璃中制备该溶液，因为染料可能会吸附到玻璃表面。 为了获得最佳结果，应在准备后的几个小时内使用此溶液。
实验步骤
根据DNA样品的估计浓度，样品量可以在1?20 uL的范围内。推荐的样品量为10 uL，DNA浓度在0.2?100 ng / uL范围内。如果使用其他样品体积，请在浓度计算中调整稀释倍数。
10 uL样品体积（DNA浓度在0.2?100 ng / uL范围内）实验方案。
标准校准曲线的准备
对于Portelite 分析，您可以选择使用DNA标准液绘制校正曲线。
图示
图1.使用具有宽动态范围的Portelite 荧光DNA定量试剂盒生成的DNA标准曲线。 使用绿色荧光通道定量荧光强度，使用线性拟合计算回归模型。 |
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