产品名称：Gelite 橙色核酸凝胶染色试剂盒

规格：1kit

储存条件：保存在冰箱-15℃避光干燥

保质期：12个月

产品物理化学光谱特性

外观：液体

激发波长（nm）：495

发射波长（nm）：540

产品介绍

Gelite 橙色核酸凝胶染色试剂盒 是美国AAT Bioquest生产的核酸染料 ，Cyber Orange 是一种极其敏感的核酸凝胶染料，可使用标准的300 nm UV透照仪和Polaroid 667黑白印刷胶片检测凝胶中的DNA或RNA。 与Cyber Green 染色剂一样，这种出色的敏感性可以归因于独特的染料特性的结合。 由于与核酸结合的Cyber Orange 染料在?495 nm和?300 nm处均显示出最大激发光（最大发射量为?537 nm），因此它可与多种仪器兼容，包括紫外线落射照明器和透射照明器以及 蓝光透射灯，以及基于汞弧灯和氩离子激光的凝胶扫描仪。我们的Gelite 橙色核酸凝胶染色凝胶试剂盒包括我们的Cyber Orange 核酸染色剂，具有优化且稳定的操作流程。 它为凝胶中的核酸样品染色提供了方便的操作方案。

样品实验方案

溶液配制

工作溶液

将1μLGelite 橙色（组分A）添加到200μL5X凝胶上样缓冲液（组分B）中。 通过用箔纸覆盖或将其置于黑暗中来保护工作溶液免受光照。

操作步骤

1.根据需要准备DNA样品。

2.将4 μL Gelite Orange工作溶液加入16 μL DNA样品中，并充分混合。 电泳前，在室温下孵育5-15分钟。

3.根据您的标准方案运行凝胶。

4.使用300 nm紫外线或254 nm透照器或使用长路径绿色滤光片（例如SYBR®滤光片或GelStar®滤光片）的基于激光的凝胶扫描仪对凝胶成像。

参考文献

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
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Journal: PLoS One (2014): e98108

A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus
Authors: Abera T, Thangavelu A, Joy Chandran ND, Raja A.
Journal: BMC Vet Res (2014): 22

A SYBR-green I quantitative real-time reverse transcription-PCR assay for rabies viruses with different virulence
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Journal: Virol Sin (2014): 131

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
Authors: Tajadini M, Panjehpour M, Javanmard SH.
Journal: Adv Biomed Res (2014): 85

Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA
Authors: Ceccarelli M, Galluzzi L, Migliazzo A, Magnani M.
Journal: PLoS One (2014): e88845

Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR
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Journal: Virusdisease (2014): 137

Development and comparative evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of West Nile virus in human patients
Authors: Kumar JS, Saxena D, Parida M.
Journal: Mol Cell Probes. (2014)

Development and evaluation of SYBR Green-I based quantitative PCR assays for herpes simplex virus type 1 whole transcriptome analysis
Authors: Garvey CE, McGowin CL, Foster TP.
Journal: J Virol Methods (2014): 101

Development of a High-resolution Melting Analysis Method Based on SYBR Green-I for rs7216389 Locus Genotyping in Asthmatic Child Patients
Authors: Vali Z, Raz A, Bokharaei H, Nabavi M, Bemanian MH, Yazdi MS, Djadid ND.
Journal: Avicenna J Med Biotechnol (2014): 72

Development of a SYBR Green I based one-step real-time PCR assay for the detection of Hantaan virus
Authors: Jiang W, Wang PZ, Yu HT, Zhang Y, Zhao K, Du H, Bai XF.
Journal: J Virol Methods (2014): 145