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| 产地 | American |
| 品牌 | AAT Bioquest |
| 货号 | CS17288 |
| 用途 | Research Grade |
| 包装规格 | 1ml |
| 纯度 | 98%% |
| CAS编号 | |
| 是否进口 | 是 |
产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*
规格：1ml
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
溶剂：水
产品介绍
用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液不含 ROX 参考染料。
适用仪器
| qPCR | |
| 仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
| 成分 | 体积 (25 μL/reaction) | 体积 (50 μL/reaction) | 最终浓度 |
| TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* | 12.5 μL | 25 μL | 1X |
| 上游引物，10 μM | 0.25-2.5 μL | 0.5-5.0 μL | 0.1-1.0 μM |
| 下游引物，10 μM | 0.25-2.5 μL | 0.5-5.0 μL | 0.1-1.0 μM |
| DNA模板 | 1-5 μL | 1-5 μL | 优化的浓度 |
| 无核酸酶水 | 25 μL | 50 μL |
表 2. 热循环参数
| 范围 | 聚合酶激活 | PCR (30-40个循环) | ||
| Hold | 变性 | 退火 | 延伸 | |
| 温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
| 时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291
Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621
Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99
A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564
A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333
BlueTYPE - A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881
Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636
Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645
One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618
Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291
