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| 产地 | American |
| 品牌 | AAT Bioquest |
| 货号 | CS17292 |
| 用途 | Research Grade |
| 包装规格 | 1 mL |
| 纯度 | 98%% |
| CAS编号 | |
| 是否进口 | 是 |
产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*
规格：1ml
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
溶剂：水
产品介绍
用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液含大量 ROX 参考染料。
适用仪器
| qPCR | |
| 仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
| 成分 | 体积 (25 μL/reaction) | 体积 (50 μL/reaction) | 最终浓度 |
| TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* | 12.5 μL | 25 μL | 1X |
| 上游引物，10 μM | 0.25-2.5 μL | 0.5-5.0 μL | 0.1-1.0 μM |
| 下游引物，10 μM | 0.25-2.5 μL | 0.5-5.0 μL | 0.1-1.0 μM |
| DNA模板 | 1-5 μL | 1-5 μL | 优化的浓度 |
| 无核酸酶水 | 25 μL | 50 μL |
表 2. 热循环参数
| 范围 | 聚合酶激活 | PCR (30-40个循环) | ||
| Hold | 变性 | 退火 | 延伸 | |
| 温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
| 时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
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Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
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Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
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Journal: BioTechniques (2014): 151-5
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[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
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