荧光底物Xite Green β-D-半乳糖吡喃糖苷*绿色荧光*
物品单位 价格 品牌
2472 AAT Bioquest
  • 产地:American
  • 型号:1 mg
  • 货号:CS14030
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-23
产地 American
品牌 AAT Bioquest
货号 CS14030
用途范围 Research Grade
纯度 98%%
规格 1 mg

Xite Green β-D-半乳糖吡喃糖苷是β-半乳糖苷酶(β-gal)的荧光底物。与现有的β-半乳糖苷酶底物(例如,常用的FDG)相比,它具有更好的细胞通透性。Xite Green β-D-吡喃半乳糖苷很容易进入细胞,并被β-gal裂解,从而产生强荧光产品Xite Green。强烈荧光的Xite Green可以很好地保留在细胞中,从而易于通过流式细胞仪和荧光显微镜进行检测。Xite Greenβ-D-半乳糖吡喃糖苷提供了一种简单而灵敏的工具来检测β-半乳糖苷酶的活性。Xite Greenβ-D-吡喃半乳糖苷可以作为检测细胞中细胞衰老的工具,因为β-gal已被确定为细胞衰老的可靠标记。


激发: 488nm激光
发射: 530/30 nm滤波片
通道: FITC滤波片组
激发: FITC滤波片
发射: FITC滤波片
推荐孔板: 黑色透明





  1. 根据需要处理样品
  2. 准备Xite Green β-D-吡喃半乳糖苷工作溶液并将其添加到样品中
  3. 在37°C下孵育样品15至45分钟
  4. 使用带有530/30 nm滤光片的流式细胞仪(FITC通道)或带有FITC滤光片组的荧光显微镜检测荧光强度



Xite Greenβ-D-吡喃半乳糖苷储备溶液:在Xite Green β-D-吡喃半乳糖苷中加入适量的DMSO,制成2-5 mM Xite Green β-D-吡喃半乳糖苷原液。注意:将未使用的Xite Green β D-吡喃半乳糖苷原液以等份储存在-20°C下。
Xite Green β-D-吡喃半乳糖苷工作溶液:在自备的缓冲液中制备1-20 μM Xite Green β-D-吡喃半乳糖苷工作溶液。注意1:Xite Green β-D-吡喃半乳糖苷工作溶液应立即使用。注意2:Xite Green β-D-吡喃半乳糖苷的浓度应针对不同的细胞类型和条件进行优化。


  1. 根据需要处理样品。
  2. 处理并用自备的缓冲液(例如DPBS)洗涤细胞。
  3. 加入Xite Green β-D-吡喃半乳糖苷工作溶液15-45分钟,然后在37°C的培养箱中培养样品。
  4. 取出工作溶液并用自备的缓冲液洗涤细胞。
  5. 将细胞重悬在自备的缓冲液中,并使用流式细胞仪使用530/30 nm滤光片(FITC通道)或带有FITC滤光片组的荧光显微镜检测荧光强度。


图1.用Xite Green β-D-吡喃半乳糖苷测量β-gal的表达。将9L-LacZ细胞(过度表达β-gal的细胞)与Xite Green β-D-半乳糖吡喃糖苷或FDG在37°C孵育30分钟。使用NovoCyte流式细胞仪(ACEA Biosciences)通过FITC通道获取信号。


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