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产地 | American |
品牌 | AAT Bioquest |
货号 | CS22857 |
用途 | Research Grade |
包装规格 | 25 Tests |
纯度 | 98%% |
CAS编号 | |
是否进口 | 是 |
Cell Meter TUNEL细胞凋亡测定试剂盒是一种强大的工具,可方便地检测由DNA的片段化引起的细胞凋亡。该测定是非放射性且快速的。TUNEL分析使用末端脱氧核苷酸转移酶(TdT)催化DEAC-dUTP在片段DNA的游离3'-羟基末端的掺入。通过荧光显微镜(AMC滤波片组)分析所得的DEAC标记的DNA。它的蓝色激发光可以方便地与GFP标记的靶标多路复用。荧光DEAC标记的核苷酸的直接掺入可明显减少检测步骤。该试剂盒经过优化,可检测固定细胞和福尔马林固定,石蜡包埋的组织切片中的凋亡。
适用仪器
流式细胞仪 | |
激发: | 405nm激光 |
发射: | 525/50nm |
通道: | Pacific Orange通道 |
荧光显微镜 | |
激发: | 紫色滤波片组 |
发射: | 紫色滤波片组 |
推荐孔板: | 黑色透明 |
样品分析方案
概述
根据需要处理样品
在冰上用4%甲醛溶液固定细胞30分钟
在冰上用70%冰冷的乙醇透化细胞60分钟
将TdT染色溶液添加到样品中并在37°C下孵育60分钟
使用带有Violet滤光片组的荧光显微镜检测荧光强度
工作溶液配制
操作步骤
以下方案可用作参考,实际情况应根据需要进行调整。
以下方案可用作参考,实际应根据需要进行调整。
去石蜡和水化
固定
染色
图示
图1.用 HeLa细胞进行TUNEL分析的荧光图像。 固定HeLa细胞,并在37°C下分别使用和不使用DNAse处理60分钟。然后将细胞用Cell Meter™固定细胞和组织TUNEL细胞凋亡测定试剂盒(Cat#CS22855 )染色。DNA链断裂在DNAse处理的细胞中表现出强烈的荧光染色。使用紫色滤光片组在荧光显微镜下检测信号。
参考文献
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