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iFluor 440-dUTP
物品单位 价格 品牌
1200 AAT Bioquest
  • 产地:American
  • 型号:25 nmoles
  • 货号:CS17029
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-24
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS17029
用途范围 Research Grade
纯度 98%%
CAS编号
规格 25 nmoles
是否进口

产品名称:iFluor 440-dUTP

规格:25 nmoles

储存条件:-15℃避光防潮

保质期:24个月

产品物理化学光谱特性

分子量:994.73

溶剂:水

吸收波长(nm):430

激发波长(nm):434

发射波长(nm):480

消光系数(nm):40000

校正因子(280 nm):0.229

产品介绍

染料修饰的脱氧尿苷5'-三磷酸酯(例如氨基烯丙基-dUTP)可用于通过常规酶促掺入方法(例如逆转录,缺口翻译,随机引物标记或PCR)生产含染料的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。由于DEAC和SpectrumAqua具有非常相似的光谱特性,DEAC-dUTP缀合物(#17011)与SpectrumAqua 滤波片组(SpectrumAqua 是Vysis的商标)一起使用产生蓝色荧光色。但是,DECA荧光团的极高疏水性使DEAC缀合物易于吸附在塑料表面上,从而导致很高的测定误差。 iFluor 440染料具有出色的水溶性,可替代DEAC染料。 iFluor 440-dUTP缀合物是DEAC-dUTP缀合物的优良替代品。 iFluor 440染料缀合物比相应的DEAC生物缀合物或其他光谱相似的染料(例如SpectrumAqua)更亮。 iFluor 440荧光团的光谱与SpectrumAqua的滤波片组非常匹配(SpectrumAqua 是Vysis的商标)。


参考文献

MECOM (EVI1) Rearrangements: A Review and Case Report of Two MDS Patients with Complex 3q Inversion/Deletions.
Authors: Lawce, Helen and Szabo, Elina and Torimaru, Yumi and Davis, Craig and Osterberg, Karin and Olson, Susan and Moore, Steve
Journal: Journal of the Association of Genetic Technologists (2017): 9-14

Application of tri-colour, dual fusion fluorescence in situ hybridization (FISH) system for the characterization of BCR-ABL1 fusion in chronic myelogenous leukaemia (CML) and residual disease monitoring.
Authors: Siu, Lisa Lp and Ma, Edmond Sk and Wong, Wai Shan and Chan, Man Hong and Wong, Kit Fai
Journal: BMC blood disorders (2009): 4

Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice.
Authors: Rojo, Marcial García and Bueno, Gloria and Slodkowska, Janina
Journal: Folia histochemica et cytobiologica (2009): 349-54

Bladder cancer detection using FISH (UroVysion assay).
Authors: Halling, Kevin C and Kipp, Benjamin R
Journal: Advances in anatomic pathology (2008): 279-86

Comparison of quantitative immunofluorescence with conventional methods for HER2/neu testing with respect to response to trastuzumab therapy in metastatic breast cancer.
Authors: Giltnane, Jennifer M and Molinaro, Annette and Cheng, Huan and Robinson, Andrew and Turbin, Dmitry and Gelmon, Karen and Huntsman, David and Rimm, David L
Journal: Archives of pathology & laboratory medicine (2008): 1635-47

AQUA and FISH analysis of HER-2/neu expression and amplification in a small cell lung carcinoma tissue microarray.
Authors: Giltnane, J M and Murren, J R and Rimm, D L and King, B L
Journal: Histopathology (2006): 161-9

A novel tricolor, dual-fusion fluorescence in situ hybridization method to detect BCR/ABL fusion in cells with t(9;22)(q34;q11.2) associated with deletion of DNA on the derivative chromosome 9 in chronic myelocytic leukemia.
Authors: Smoley, Stephanie A and Brockman, Stephanie R and Paternoster, Sarah F and Meyer, Reid G and Dewald, Gordon W
Journal: Cancer genetics and cytogenetics (2004): 1-6


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