产品名称：Cy3 Bis NTA-Ni复合物

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：1356.60

溶剂：DMSO

激发波长(nm)：555

发射波长(nm)：569

产品介绍

聚组氨酸是掺入重组蛋白中最流行的亲和标签之一。它可以插入N端或C端，并在多种宿主中表达。由于其体积小，聚组氨酸标签可作为蛋白质纯化和检测的理想工具。 HIS Lite™Cy3 Bis NTA-Ni和Cy5 Bis NTA-Ni复合物可对His标记的融合蛋白进行特异性和高灵敏度的检测。 Ni-NTA配合物最早由Kapanidis等报道。对具有最小交叉反应性的多组氨酸标签具有特异性。 Cy3和Cy5染料在通常可用的波长处显示强荧光信号，几乎没有猝灭。 Cy3 Bis NTA-Ni和Cy5 Bis NTA-Ni复合物可直接应用于SDS-PAGE凝胶或Western blot膜进行荧光成像。用Cy3 Bis NTA-Ni和Cy5 Bis NTA-Ni复合物进行检测所需的孵育时间少于蛋白质-抗体结合所需的孵育时间。由于Ni-NTA络合物直接与荧光团偶联，因此不需要二次反应。

参考文献

Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Authors: de Costa F, Barber CJ, Pujara PT, Reed DW, Covello PS.
Journal: Methods Mol Biol (2016): 91

Cellular uptake and in vivo distribution of polyhistidine peptides
Authors: Iwasaki T, Tokuda Y, Kotake A, Okada H, Takeda S, Kawano T, Nakayama Y.
Journal: J Control Release (2015): 115

Characterization of soluble RNA-dependent RNA polymerase from dengue virus serotype 2: The polyhistidine tag compromises the polymerase activity
Authors: Kamkaew M, Chimnaronk S.
Journal: Protein Expr Purif (2015): 43

New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli
Authors: Schwendener S, Perreten V.
Journal: Appl Environ Microbiol (2015): 3243

Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deinococcus geothermalis
Authors: Panek A, Pietrow O, Filipkowski P, Synowiecki J.
Journal: Acta Biochim Pol (2013): 163

Electrodeposition of polymer nanodots with controlled density and their reversible functionalization by polyhistidine-tag proteins
Authors: Bazin D, Chevalier S, Saadaoui H, Santarelli X, Larpent C, Feracci H, Faure C.
Journal: Langmuir (2012): 13968

Surface-attached polyhistidine-tag proteins characterized by FTIR difference spectroscopy
Authors: Pinkerneil P, Guldenhaupt J, Gerwert K, Kotting C.
Journal: Chemphyschem (2012): 2649

A facile method for reversibly linking a recombinant protein to DNA
Authors: Goodman RP, Erben CM, Malo J, Ho WM, McKee ML, Kapanidis AN, Turberfield AJ.
Journal: Chembiochem (2009): 1551

A pair of ligation-independent Escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins
Authors: Dan H, Balach and ran A, Lin M.
Journal: J Biomol Tech (2009): 241

RNA aptamer binding to polyhistidine-tag
Authors: Tsuji S, Tanaka T, Hirabayashi N, Kato S, Akitomi J, Egashira H, Waga I, Ohtsu T.
Journal: Biochem Biophys Res Commun (2009): 227