即用型过氧化氢溶液是美国AAT Bioquest生产的ELISA检测试剂 ，与其它商业化过氧化氢溶液相比，我们制备的H₂O₂溶液更加稳定。它经过校准，能够更好的保证基于过氧化酶检测的重现性。

操作方案

1.储备溶液配制

所有未使用的储备溶液应分成一次性等分试样，并在制备后储存在-20°C。避免反复冻融循环。

1.过氧化物酶反应混合物：
用适当的过氧化氢底物浓度和50至500μM过氧化氢制备过氧化物酶反应混合物。

样品实验方案

1.加入等体积的过氧化物酶工作溶液和过氧化物酶标准品和含过氧化物酶的样品。

2.在室温下孵育反应15至30分钟，避光。

3.用适当的仪器监测荧光或吸光度。

参考文献

Development of enzyme-based bar code-style lateral-flow assay for hydrogen peroxide determination
Authors: Fung KK, Chan CP, Renneberg R.
Journal: Anal Chim Acta (2009): 89

The accuracy of Amplex Red assay for hydrogen peroxide in the presence of nanoparticles
Authors: Lee CW, Chen YC, Ostafin A.
Journal: J Biomed Nanotechnol (2009): 477

Development of a 384-well colorimetric assay to quantify hydrogen peroxide generated by the redox cycling of compounds in the presence of reducing agents
Authors: Johnston PA, Soares KM, Shinde SN, Foster CA, Shun TY, Takyi HK, Wipf P, Lazo JS.
Journal: Assay Drug Dev Technol (2008): 505

Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation
Authors: Ozyurek M, Bektasoglu B, Guclu K, Apak R.
Journal: Anal Chim Acta (2008): 196

A reporter system for the individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo
Authors: Shao N, Krieger-Liszkay A, Schroda M, Beck CF.
Journal: Plant J (2007): 475

Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS
Authors: Arakawa H, Masuda K, Tajima N, Maeda M.
Journal: Luminescence (2007): 245

Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide
Authors: Wu ZS, Zhang SB, Guo MM, Chen CR, Shen GL, Yu RQ.
Journal: Anal Chim Acta (2007): 122

Polypyrrole-based optical probe for a hydrogen peroxide assay
Authors: Wang H, Park SM.
Journal: Anal Chem (2007): 240

Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b6f complex: a simple and effective assay for stability of the complex in detergent solutions
Authors: Chen XB, Zhao XH, Zhu Y, Gong YD, Li LB, Zhang JP, Kuang TY.
Journal: Photosynth Res (2006): 205

The responses of lymphocytes from Asian and Caucasian diabetic patients and non-diabetics to hydrogen peroxide and sodium nitrite in the Comet assay
Authors: Wyatt N, Kelly C, Fontana V, Merlo DF, Whitelaw D, Anderson D.
Journal: Mutat Res (2006): 154