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MegaWox polyHRP-蛋白质缀合物
物品单位 价格 品牌
4992 AAT Bioquest
  • 产地:American
  • 型号:1 mg
  • 货号:CS11041
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS11041
用途范围 Research Grade
纯度 98%%
CAS编号
规格 1 mg
是否进口

产品名称:MegaWox polyHRP-蛋白质缀合物

规格:1mg

储存条件:保存在冰箱-15℃干燥

保质期:12个月


产品介绍
 

蛋白A是在金黄色葡萄球菌细胞壁中发现的42 kDa蛋白。它以高亲和力结合各种物种的免疫球蛋白的Fc区。抗体有四个结合位点,但只能同时使用其中两个。蛋白A缀合物可用于间接检测固定在固体支持物或组织样品中的抗原。在这些测定中,将未标记的检测抗体添加到固相中,然后用蛋白A缀合物对形成的免疫复合物进行定量。如果使用Fab片段代替整个抗体分子包被固相,则蛋白A结合物可用于夹心式免疫测定中的免疫复合物的间接检测。蛋白A对免疫球蛋白的亲和力取决于免疫球蛋白的种类。例如,小鼠IgG1单克隆抗体的亲和力低,人和兔多克隆抗体的亲和力高。MegaWox polyHRP-Protein A缀合物在样品量有限或目标分子含量低的免疫分析中提供最高的灵敏度和低背景。如果使用FAB片段代替整个抗体分子包被固相,则蛋白A结合物可用于夹心式免疫检测中的免疫复合物的间接检测。蛋白A对免疫球蛋白的亲和力取决于免疫球蛋白的种类。例如,小鼠IgG1

 

参考文献

A New Approach for Affinity-Based Purification of Horseradish Peroxidase.
Authors: Almaz, Zuleyha and Oztekin, Aykut and Abul, Nurgul and Gerni, Serpil and Erel, Deniz and Kocak, Seyma Mehtap and Sengül, Mehmet Emin and Ozdemir, Hasan
Journal: Biotechnology and applied biochemistry (2020)

A novel bioassay for the monitoring of hydrogen peroxide in human plasma samples based on binding of horseradish peroxidase-conjugated prostate specific antigen to poly (toluidine blue) as imprinted polymer receptor.
Authors: Sardaremelli, Sanam and Razmi, Habib and Hasanzadeh, Mohamad and Shadjou, Nasrin
Journal: International journal of biological macromolecules (2020): 311-324

Acetylcholinesterase biosensors based on ionic liquid functionalized carbon nanotubes and horseradish peroxidase for monocrotophos determination.
Authors: Jiaojiao, Xia and Bin, Zou and Pengyun, Wang and Qing, Luo and Xin, Shen and Rui, Ji
Journal: Bioprocess and biosystems engineering (2020): 293-301

Amperometric Flow-Injection Analysis of Phenols Induced by Reactive Oxygen Species Generated under Daylight Irradiation of Titania Impregnated with Horseradish Peroxidase.
Authors: Rahemi, Vanoushe and Trashin, Stanislav and Hafideddine, Zainab and Van Doorslaer, Sabine and Meynen, Vera and Gorton, Lo and De Wael, Karolien
Journal: Analytical chemistry (2020): 3643-3649

Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356

Bioanalytical Application of Peroxidase-Mimicking DNAzymes: Status and Challenges.
Authors: Kosman, J and Juskowiak, B
Journal: Advances in biochemical engineering/biotechnology (2020): 59-84

Development of a Surfactant-Containing Process to Improve the Removal Efficiency of Phenol and Control the Molecular Weight of Synthetic Phenolic Polymers Using Horseradish Peroxidase in an Aqueous System.
Authors: Yao, Chao-Ling and Lin, Che-Chi and Chu, I-Ming and Lai, Yi-Ting
Journal: Applied biochemistry and biotechnology (2020)

Development of a new and simple method for the detection of histidine-tagged proteins based on thionine-chitosan/gold nanoparticles/horseradish peroxidase.
Authors: Ren, Ruijuan and Lu, Dingqiang and Pang, Guangchang
Journal: Biomedical microdevices (2020): 11

Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme: Principle and Biosensing Application.
Authors: Alizadeh, Negar and Salimi, Abdollah and Hallaj, Rahman
Journal: Advances in biochemical engineering/biotechnology (2020): 85-106

High peroxidase-like activity realized by facile synthesis of FeS2 nanoparticles for sensitive colorimetric detection of H2O2 and glutathione.
Authors: Song, Chan and Ding, Wei and Zhao, Weiwen and Liu, Haibo and Wang, Jie and Yao, Yuewei and Yao, Cheng
Journal: Biosensors & bioelectronics (2020): 111983

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