15926423062  

产品展厅

Amplite 比色法Bradford蛋白定量分析试剂盒
物品单位 价格 品牌
1200 AAT Bioquest
  • 产地:American
  • 型号:1000 Tests
  • 货号:CS11118
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS11118
用途范围 Research Grade
纯度 98%%
CAS编号
规格 1000 Tests
是否进口
简要概述

传统的Bradford蛋白质检测法广泛用于定量蛋白质浓度。但是,许多市场的许多操作说明非常复杂繁琐。 我们的Amplite 比色法Braford蛋白定量检测试剂盒是一种用于测定总蛋白浓度的包含两组分且与洗涤剂兼容的测定方法。该检测法基于与Bradford蛋白质测定法相同的考马斯亮蓝G-250蛋白指示剂,并提供相当的准确性。我们专有的配方使我们的试剂盒更加方便快捷。在600 nm附近检测蛋白质信号,并在30分钟内完成测定。 Amplite 比色'Braford蛋白定量分析试剂盒可以以方便的96孔板进行操作,无需分离步骤即可轻松实现自动化。


适用仪器


光吸收酶标仪
吸收: 595nm
推荐孔板: 透明底板


产品说明书

样品分析方案

概述

准备Bradford工作溶液(50μL)
添加BSA标准品或检测样品(50μL)
在室温下孵育5-15分钟
读取595 nm处的吸光度

注:使用前,请在室温下解冻所有试剂盒组分。

标准溶液制备:

BSA标准溶液:

将20 μL的1 mg / mL BSA标准溶液(组分C)添加到480 μL的PBS(未提供)中,以生成40 μg / mL的BSA标准溶液(BS1)。然后在PBS中执行1:2连续稀释,以得到连续稀释的BSA标准BS2-BS7。注意:有必要为每个测定创建标准曲线。

操作步骤

表1.在透明的底96孔板中布置BSA标准品和测试样品。 BS = BSA标准品(BS1-BS7,40至0.625 μg / mL); BL =空白控件; TS =测试样品

BS1 BS1 TS TS
BS2 BS2 ... ...
BS3 BS3
BS4 BS4
BS5 BS5
BS6 BS6
BS7 BS7
BL BL

表2:每个孔的试剂

溶液体积 试剂
BS1-BS7 50 μL 系列稀释液(40-0.625 μg / mL)
BL 50 μL PBS
TS 50 μL 测试样品

1.根据表1和表2提供的布局准备BSA标准(BS),空白对照(BL)和检测样品(TS)。
2.向BSA标准品,空白对照和测试样品的每个孔中添加50 μL的Bradford工作溶液,以使总测定体积为100 μL /孔。
3.将反应在室温下孵育5至15分钟。
4.用酶标仪在OD 595 nm处读取吸光度。


图示

图1.使用透明底96孔板,使用Amplite 比色法Bradford蛋白定量分析试剂盒测量BSA剂量响应。

参考文献

The Effects of a Concentrated Surfactant Gel on Biofilm EPS.
Authors: Salisbury, Anne-Marie and Chen, Rui and Mullin, Marc and Foulkes, Lauren and Percival, Steven L
Journal: Surgical technology international (2020): 31-35

The effect of Chinese wild blueberry fractions on the growth and membrane integrity of various foodborne pathogens.
Authors: Zhou, Tong-Tong and Wei, Cai-Hong and Lan, Wei-Qing and Zhao, Yong and Pan, Ying-Jie and Sun, Xiao-Hong and Wu, Vivian C H
Journal: Journal of food science (2020): 1513-1522

[Preliminary Investigation on Difference of Protein Compositions Between DC2.4 Cells and Their Derived Exosomes by nanoLC-MS/MS].
Authors: Lin, Qing and Li, Yan and Qu, Meng-Ke and Liu, Xing and Zhang, Zhi-Rong and Zhang, Ling
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2020): 81-86

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions.
Authors: Zendrini, Andrea and Paolini, Lucia and Busatto, Sara and Radeghieri, Annalisa and Romano, Miriam and Wauben, Marca H M and van Herwijnen, Martijn J C and Nejsum, Peter and Borup, Anne and Ridolfi, Andrea and Montis, Costanza and Bergese, Paolo
Journal: Frontiers in bioengineering and biotechnology (2019): 452

Combined Fluorimetric Caspase-3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity.
Authors: Larsen, Anna K and Hall, Arnaldur and Lundsgart, Henrik and Moghimi, S Moein
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 301-311

Effect of nanoparticle size and PEGylation on the protein corona of PLGA nanoparticles.
Authors: Partikel, Katrin and Korte, Robin and Stein, Nora C and Mulac, Dennis and Herrmann, Fabian C and Humpf, Hans-Ulrich and Langer, Klaus
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2019): 70-80

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay.
Authors: Khramtsov, Pavel and Barkina, Irina and Kropaneva, Maria and Bochkova, Maria and Timganova, Valeria and Nechaev, Anton and Byzov, Il'ya and Zamorina, Svetlana and Yermakov, Anatoly and Rayev, Mikhail
Journal: Nanomaterials (Basel, Switzerland) (2019)

Pilose Antler Extracts (PAEs) Protect against Neurodegeneration in 6-OHDA-Induced Parkinson's Disease Rat Models.
Authors: Li, Chaohua and Sun, Yanan and Yang, Weifeng and Ma, Shuhua and Zhang, Lili and Zhao, Jing and Zhao, Xin and Wang, Yi
Journal: Evidence-based complementary and alternative medicine : eCAM (2019): 7276407

Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies.
Authors: Wu, Di and Xue, Xiaowei and Gao, Chenchen and Liu, Yuehong and Wang, Tiantian and Li, Lisheng and Tong, Xuehong and Li, Feng and Xu, Jingdong
Journal: Scientific reports (2019): 19375

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles.
Authors: Partikel, Katrin and Korte, Robin and Mulac, Dennis and Humpf, Hans-Ulrich and Langer, Klaus
Journal: Beilstein journal of nanotechnology (2019): 1002-1015


< 返回前一个页面 >





联系方式
手机:15926423062
电话:15926423062
手机访问官网

15926423062

武汉科斯坦生物科技有限公司 版权所有(C)2022  XML 技术支持: 食品商务网   盖德化工网  

武汉科斯坦生物科技有限公司