产品名称：小沟结合物探针CDPI3-CPG*500A*

规格：100 mg

储存条件：-20℃干燥

保质期：12个月

产品物理化学光谱特性

外观：固体

溶剂：MeCN

产品介绍

小沟结合物（MGB）分子选择性地结合到DNA分子的小沟，即DNA螺旋中的浅沟。MGB标记的寡核苷酸与互补的DNA和RNA靶序列形成稳定的杂合复合物。天然产物CC-1065的类似物二氢吡咯并吲哚三肽（CDPI3）已表现出与寡核苷酸连接时可在单链DNA上阻止序列特异性引物延伸。包含CDPI3的寡核苷酸可提高特异性和杂交强度，从而增强DNA双链体的稳定性。将MGB分子添加到DNA探针可以设计出较短的杂交探针，该探针可用于分子诊断，例如基于定量PCR的测定。可能有几种设计，包括Taqman MGB探针。AAT Bioquest提供此CPG支持，可将MGB部分方便地添加到寡核苷酸的3'末端。我们还提供MGB亚磷酰胺，用于用MGB部分标记5'末端。

参考文献

Oligonucleotide-minor groove binder conjugates and their complexes with complementary DNA: effect of conjugate structural factors on the thermal stability of duplexes.
Authors: Ryabinin, Vladimir A and Boutorine, Alexandre S and Hélène, Claude and Pyshnyi, Dmitrii V and Sinyakov, Alexandre N
Journal: Nucleosides, nucleotides & nucleic acids (2004): 789-803

The preferential binding of histone H1 to DNA scaffold-associated regions is determined by its C-terminal domain.
Authors: Roque, Alicia and Orrego, Mary and Ponte, Imma and Suau, Pedro
Journal: Nucleic acids research (2004): 6111-9

Synthesis and evaluation of oligo-1,3-thiazolecarboxamide derivatives as HIV-1 reverse transcriptase inhibitors.
Authors: Ryabinin, V A and Zakharova, O D and Yurchenko, E Y and Timofeeva, O A and Martyanov, I V and Tokarev, A A and Belanov, E F and Bormotov, N I and Tarrago-Litvak, L and Andreola, M L and Litvak, S and Nevinsky, G A and Sinyakov, A N
Journal: Bioorganic & medicinal chemistry (2000): 985-93

[Effect of structural factors on the stability of duplexes formed by oligonucleotide conjugates with minor groove ligands].
Authors: Riabinin, V A and Butorin, A S and Elen, K and Denisov, A Iu and Pyshny?, D V and Siniakov, A N
Journal: Bioorganicheskaia khimiia: 159-66