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FAM-xtra 亚磷酰胺
物品单位 价格 品牌
1164 AAT Bioquest
  • 产地:American
  • 型号:50 umoles
  • 货号:CS6037
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS6037
用途范围 Research Grade
纯度 98%%
CAS编号
规格 50 umoles
是否进口

产品名称:FAM-xtra 亚磷酰胺

规格:50 umoles

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:879.94

溶剂:MeCN

 

产品介绍

6-FAM亚磷酰胺可能是最流行的荧光构建基块,已广泛用于通过荧光素荧光团有效标记5'端的寡核苷酸。标准裂解和氢氧化铵脱保护用于释放荧光素标记的寡核苷酸。尽管6-FAM已普遍用于开发各种qPCR探针,但其荧光受到两个作用限制:1.高度依赖pH值;2.光漂白迅速。目前我们已开发出FAM-xtra 亚磷酰胺解决了这两个局限性,同时完整保留了6-FAM亚磷酰胺的所有优点。 FAM-xtra亚磷酰胺可在相同操作条件下与6-FAM亚磷酰胺完全一样使用,而无需进行任何更改。由FAM-xtra亚磷酰胺制成的寡核苷酸具有许多优点:1.FAM-xtra标记的寡核苷酸具有与6-FAM标记的寡核苷酸相同的光谱。 2.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸明亮得多。 3.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸具有更高的光稳定性。4.与相应的FAM标记的寡核苷酸相比,FAM-xtra标记的寡核苷酸的荧光对pH的敏感度低得多。在生理条件下,FAM-xtra标记的寡核苷酸的荧光基本上与pH无关,这使得FAM-xtra标记的寡核苷酸的qPCR探针更加稳定。

 

参考文献

Development of an Innovative Intradermal siRNA Delivery System Using a Combination of a Functional Stearylated Cytoplasm-Responsive Peptide and a Tight Junction-Opening Peptide.
Authors: Ibaraki, Hisako and Kanazawa, Takanori and Takashima, Yuuki and Okada, Hiroaki and Seta, Yasuo
Journal: Molecules (Basel, Switzerland) (2016)

Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling.
Authors: Kvach, Maksim V and Tsybulsky, Dmitry A and Shmanai, Vadim V and Prokhorenko, Igor A and Stepanova, Irina A and Korshun, Vladimir A
Journal: Current protocols in nucleic acid chemistry (2013): Unit 4.55

Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes.
Authors: Kvach, Maksim V and Stepanova, Irina A and Prokhorenko, Igor A and Stupak, Aleksander P and Bolibrukh, Dmitry A and Korshun, Vladimir A and Shmanai, Vadim V
Journal: Bioconjugate chemistry (2009): 1673-82

Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotide phosphorothioates.
Authors: Karskela, Marika and Virta, Pasi and Malinen, Melina and Urtti, Arto and L?nnberg, Harri
Journal: Bioconjugate chemistry (2008): 2549-58

Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis.
Authors: Dobson, Neil and McDowell, David G and French, David J and Brown, Lynda J and Mellor, John M and Brown, Tom
Journal: Chemical communications (Cambridge, England) (2003): 1234-5

Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
Authors: Ramírez-Vick, J E and García, A A and Lee, J
Journal: Preparative biochemistry & biotechnology (1998): 243-60

Fluorescent dye phosphoramidite labelling of oligonucleotides.
Authors: Theisen, P and McCollum, C and Andrus, A
Journal: Nucleic acids symposium series (1992): 99-100

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