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AF 488醛
物品单位 价格 品牌
4992 AAT Bioquest
  • 产地:American
  • 型号:5 mg
  • 货号:CS9015
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS9015
用途范围 Research Grade
纯度 98%%
CAS编号
规格 5 mg
是否进口

产品名称:AF 488醛

规格:5 mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:639.02

溶剂:DMSO

激发波长(nm):499

发射波长(nm):520

消光系数(cm-1 M-1):73000

荧光量子产率:0.92

校正因子 (280 nm):0.11

 

产品介绍

AF488醛包含AlexaFluor®488的荧光团。它是一种可与胺,肼或羟胺反应的活性绿色荧光染料。醛基可与pH 5-9的胺,酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基(NHS)不同,醛可以在酸性pH下与N端胺基反应,这是某些生物共轭反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记,使其靶向分子上的所需位置。可以通过普通的荧光仪器在FITC通道下轻松检测到共轭AF488染料。 AlexaFluor®488是ThermoFisher的商标。

 

参考文献

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Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
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Raman nanoparticle probes for antibody-based protein detection in tissues.
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Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9

Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8

Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
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Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
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Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
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Journal: The Journal of biological chemistry (2003): 38935-41

Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
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Journal: Cytometry (2000): 316-20

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