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AF488 醛 *与 Alexa Fluor™ 488 醛的结构相同*
物品单位 价格 品牌
3612 AAT Bioquest
  • 产地:American
  • 型号:1 mg
  • 货号:CS9016
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS9016
用途范围 Research Grade
纯度 98%%
CAS编号
规格 1 mg
是否进口

产品名称:AF488 醛 *与 Alexa Fluor™ 488 醛的结构相同*

规格:1mg

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:750.75

外观:固体

溶剂:DMSO

激发波长(nm):499

发射波长(nm):520

 

产品介绍

AF488 由 AAT Bioquest 生产,与 Alexa Fluor® 488 具有相同的化学结构(Alexa Fluor® 是 ThermoFisher 的商标)。 AF488 醛含有 XFD488 的荧光团。 它是一种活性绿色荧光染料,可与胺、肼或羟胺反应。 醛基对 pH 5-9 的胺、酰肼或羟胺基具有反应性。 与胺反应性琥珀酰亚胺酯基团 (NHS) 不同,醛可以在酸性 pH 下与 N 端胺基反应,某些生物偶联反应有时需要这种条件。 醛与胺基反应形成中间席夫键。 用氢化物进一步还原将形成稳定的 C-N 键。 醛和其他基团之间的反应允许荧光素染料在目标分子上的所需位置进行位点特异性结合和标记。  AF488 染料可以通过 FITC 通道下的普通荧光仪器轻松检测。

 

参考文献

Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.
Authors: Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan
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Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
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Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding.
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Raman nanoparticle probes for antibody-based protein detection in tissues.
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Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8

Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
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Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
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Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
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Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
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Journal: Cytometry (2000): 316-20

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