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化学磷酸化试剂I (CPR I)
物品单位 价格 品牌
6564 AAT Bioquest
  • 产地:American
  • 型号:1 g
  • 货号:CS6001
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS6001
用途范围 Research Grade
纯度 98%%
CAS编号
规格 1 g
是否进口

产品名称:化学磷酸化试剂I (CPR I)

规格:1g

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:656.77

溶剂:MeCN

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

这种试剂可用来产生3’-磷酸化,这被看做是第一次加成。在合成的时候,单核苷酸氨脱保护后,3’-磷酸化部位会与第2个碱基相连。载体碱基和CPR都被剪切。在氢氧化铵脱保护期间,DMT-基团被移去;因此不可用于poly-pak的纯化。

 

参考文献

Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate
Authors: Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD.
Journal: J Am Chem Soc (2006): 885

O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method
Authors: Ohkubo A, Ezawa Y, Seio K, Sekine M.
Journal: J Am Chem Soc (2004): 10884

Synthesis and conformational properties of oligonucleotides incorporating 2'-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates
Authors: Tsuruoka H, Shohda K, Wada T, Sekine M.
Journal: J Org Chem (2000): 7479

Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides
Authors: Olejnik J, Krzymanska-Olejnik E, Rothschild KJ.
Journal: Nucleic Acids Res (1996): 361

A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase
Authors: Mora N, Lacombe JM, Pavia AA.
Journal: Int J Pept Protein Res (1995): 53

Chemical synthesis of O-thiophosphotyrosyl peptides
Authors: Kitas E, Kung E, Bannwarth W.
Journal: Int J Pept Protein Res (1994): 146

Synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate
Authors: Boumendjel A, Miller SP.
Journal: J Lipid Res (1994): 2305

Syntheses of D-myo-inositol 1,4,5-trisphosphate affinity ligands
Authors: Tegge W, Ballou CE.
Journal: Carbohydr Res (1992): 63

Fmoc/solid-phase synthesis of Tyr(P)-containing peptides through t-butyl phosphate protection
Authors: Perich JW, Reynolds EC.
Journal: Int J Pept Protein Res (1991): 572


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