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ReadiUse TCEP 去除缓冲液
物品单位 价格 品牌
2604 AAT Bioquest
  • 产地:American
  • 型号:1 ml
  • 货号:CS5540
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-25
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS5540
用途范围 Research Grade
纯度 98%%
CAS编号
规格 1 ml
是否进口

产品名称:ReadiUse TCEP 去除缓冲液

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

TCEP是一种常用的还原剂,广泛用于破坏蛋白质内和蛋白质之间的二硫键。 与其他两种最常见的还原剂,即二硫苏糖醇(DTT)和2-巯基乙醇相比,TCEP是无味,更强力的还原剂,不可逆还原剂,更亲水,更耐空气氧化的优点。TCEP还用于RNA分离的组织匀浆过程。 然而,有许多报道称TCEP与通常具有对TCEP反应的组分的下游测定不相容。 我们的ReadiUse TCEP去除缓冲液可有效用于消除残留的TCEP。 这是一种预先格式化的2M解决方案,可以简单地添加到包含残余TCEP的解决方案中。

 

参考文献

A high-throughput hydrophilic interaction liquid chromatography coupled with a charged aerosol detector method to assess trisulfides in IgG1 monoclonal antibodies using tris(2-carboxyethyl)phosphine reaction products: Tris(2-carboxyethyl)phosphine-oxide and tris(2-carboxyethyl)phosphine-sulfide
Authors: Cornell C, Karanjit A, Chen Y, Jacobson F.
Journal: J Chromatogr A (2016): 107

Chemical Etching of Bovine Serum Albumin-Protected Au25 Nanoclusters for Label-Free and Separation-Free Ratiometric Fluorescent Detection of Tris(2-carboxyethyl)phosphine
Authors: Shu T, Wang J, Su L, Zhang X.
Journal: Anal Chem (2016): 11193

Palladium(II) complexes with tris(2-carboxyethyl)phosphine, structure, reactions and cytostatic activity
Authors: Pruchnik H, Lis T, Latocha M, Zielinska A, Pruchnik FP.
Journal: J Inorg Biochem (2016): 14

The Role of Tris(2-carboxyethyl)phosphine Reducing Agent in the Controlled Formation of alpha,omega-Alkanedithiols Monolayers on Au(111) with Monocoordinated and Bicoordinated Configurations
Authors: Euti EM, Velez-Romero P, Leiva EP, Macagno VA, Paredes-Olivera PA, Patrito EM, Cometto FP.
Journal: Langmuir (2016): 9428

Dissociation and reduction of covalent beta-lactoglobulin-quinone adducts by dithiothreitol, tris(2-carboxyethyl)phosphine, or sodium sulfite
Authors: Jongberg S, Lund MN, Otte J.
Journal: Anal Biochem (2015): 40

Direct measurement of active thiol metabolite levels of clopidogrel in human plasma using tris(2-carboxyethyl)phosphine as a reducing agent by LC-MS/MS
Authors: Park JB, Bae SH, Jang SM, Noh WJ, Hong JH, Yoon KD, Kang HC, Bae SK.
Journal: J Sep Sci (2013): 2306

Loss of cationic peptides with agarose gel-immobilized tris[2-carboxyethyl]phosphine (TCEP)
Authors: Shriver-Lake LC, North SH, Rowe Taitt C.
Journal: Biotechniques (2013): 292

Microwave-mediated reduction of disulfide bridges with supported (tris(2-carboxyethyl)phosphine) as resin-bound reducing agent
Authors: Miralles G, Verdie P, Puget K, Maurras A, Martinez J, Subra G.
Journal: ACS Comb Sci (2013): 169

Tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein
Authors: Chen S, Jiang H, Wei K, Liu Y.
Journal: Chem Commun (Camb) (2013): 1226

On-line cleavage of disulfide bonds by soluble and immobilized tris-(2-carboxyethyl)phosphine using sequential injection analysis
Authors: Tzanavaras PD, Mitani C, Anthemidis A, Themelis DG.
Journal: Talanta (2012): 21


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