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生物胞素 C2 马来酰亚胺
物品单位 价格 品牌
2604 AAT Bioquest
  • 产地:American
  • 型号:5 mg
  • 货号:CS3085
  • 发布日期: 2022-03-01
  • 更新日期: 2022-03-28
产品详细说明
产地 American
品牌 AAT Bioquest
货号 CS3085
用途范围 Research Grade
纯度 98%%
CAS编号
规格 5 mg
是否进口

产品名称:生物胞素 C2 马来酰亚胺

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:494.61

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

生物胞素 C2 马来酰亚胺是美国AAT Bioquest生产的生物素类荧光探针 。生物胞素马来酰亚胺易与生物大分子硫羟基部分反应,形成硫醚偶联物,它十分稳定。生物胞素马来酰亚胺需要温和地结合条件。例如,在PH5.5通常是修饰半胱氨酸残基的最佳PH, 并且无论什么时候,尽量减少暴露在空气中溶液反应,尽可能避免硫羟化底物在空气中氧化。大多偶联物在室温下形成。然而,升高或者降低温度都可能需要特定的标记反应。生物胞素必须置于无外来硫醇的缓冲液中(6-巯基乙醇、二硫苏糖醇和巯基乙胺)反应。蛋白质或者多肽通过与不含有硫醇基团(SH)的试剂进行硫羟化反应形成生物胞素。

 

参考文献

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Journal: Brain Res Brain Res Protoc (2004): 106

Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells
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Journal: Brain Res (2002): 111

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure
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Journal: J Neurosci Methods (2002): 81

A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan
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Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier
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Journal: Brain Res (2000): 348

In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing tissue
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Journal: J Neurosci Methods (2000): 1

A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189

A radioiodinated biocytin derivative for in-vivo applications
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963

A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or neurobiotin-labeled neurons
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Journal: J Histochem Cytochem (1998): 263

Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167

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