产品名称：Cy5生物素缀合物

规格：5mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1179.49

外观：固体

溶剂：水

激发波长（nm）：649

发射波长（nm）：665

产品介绍

Cy5生物素缀合物是美国AAT Bioquest生产的Cy系列产品 ，这种双功能Cy5生物素结合物表现出优异的抗生物素蛋白结合和强荧光的特性。 就高亲和力、快速结合、与抗生物素蛋白和链霉抗生物素蛋白四聚体的非协同结合而言，其具有与天然生物素类似的抗生物素蛋白结合特性。 Cy5生物素可用于检测和定量抗生物素蛋白，链霉抗生物素蛋白或中性抗生物素蛋白的生物素结合位点。 该试剂克服了常用的生物素-4-荧光素的主要缺点，例如在水性介质中溶解性差和pH依赖性吸光度和发射。Cy5生物素是水溶性试剂，生物素和荧光标签之间的柔性PEG间隔物使与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合有关的空间位阻最小化。

参考文献

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Journal: Biochem Biophys Rep (2019): 127-131

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Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid
Authors: K. Pal
Journal: Org Lett (2018): 6425-6429

A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase
Authors: T. E. H. Bond
Journal: Microbiol Res (2017): 35-39

Labeling of Cytoskeletal Proteins in Living Cells Using Biotin Ligase Carrying a Fluorescent Protein
Authors: S. Nishi
Journal: Anal Sci (2017): 897-902

A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
Authors: W. J. Shi
Journal: Dalton Trans (2016): 17798-17806

Design and synthesis of fluorescent and biotin tagged probes for the study of molecular actions of FAF1 inhibitor
Authors: S. E. Yoo
Journal: Bioorg Med Chem Lett (2016): 1169-72

Highly Selective Fluorescent Turn-On Probe for Protein Thiols in Biotin Receptor-Positive Cancer Cells
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Journal: Anal Chem (2016): 3400-5

NeutrAvidin Functionalization of CdSe/CdS Quantum Nanorods and Quantification of Biotin Binding Sites using Biotin-4-Fluorescein Fluorescence Quenching
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Journal: Bioconjug Chem (2016): 562-8