物品单位 | 价格 | 品牌 |
---|---|---|
支 | 4584 | AAT Bioquest |
产地 | American |
品牌 | AAT Bioquest |
货号 | CS2586 |
用途范围 | Research Grade |
纯度 | 98%% |
CAS编号 | |
规格 | 1 mg |
是否进口 | 是 |
产品名称:预活化的APC-Cy5.5 Tandem ReadiUse
规格:1mg
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:N/A
溶剂:水
激发波长(nm):651
发射波长(nm):700
产品介绍
预活化的APC-Cy5.5 Tandem ReadiUse是美国AAT Bioquest生产的蛋白标记染料 ,别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。与其他藻胆蛋白一样,APC是荧光的,具有极高的吸收率和高量子效率。它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。 APC-Cy5.5是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~700nm。 AAT Bioquest提供这种预活化的APC-Cy5.5,以促进APC-Cy5.5与抗体和其他蛋白质(如链霉抗生物素蛋白和其他二级试剂)的串联结合。我们的预活化APC-Cy5.5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化APC-Cy5.5串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原再生的硫醇基团。
参考文献
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Journal: Microbiology (2001): 3171
Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
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